Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/62448
Type: Artigo de periódico
Title: DOMINANT AND RECESSIVE MUTATIONS CONFERRING RESISTANCE TO S-2-AMINOETHYL-L-CYSTEINE IN MAIZE
Author: AZEVEDO, RA
ARRUDA, P
Abstract: Maize tissue culture was used to select plants resistant to the lysine analogue, S-2-aminoethyl-L-cysteine (AEC). Embryogenic calli of the maize (Zea mays L.) inbred line Cat-100-1 were mutagenized with NaN3 and selected in medium containing 0.25 mM AEC. Several plants were regenerated, transferred to soil, and grown to maturity. Two plants, AEC-1 and AEC-5, of 63 regenerated plants, when self-pollinated, segregated resistant and sensitive plants. The resistance of the AEC-1 plant proved to be determined by a single dominant gene while the resistance of AEC-5 was determined by a single recessive gene after the analysis of R1 progenies obtained from AEC-1 and AEG-5 regenerated plants. Soluble amino acids from the endosperm of both mutants were extracted and quantified by high performance liquid chromatography. Both resistant mutants presented an increased soluble lysine concentration when compared with sensitive ones.
Subject: ZEA MAYS
LYSINE
S2-AMINOETHYL-L-CYSTEINE
TISSUE CULTURE
Country: Alemanha
Editor: Elsevier Gmbh, Urban & Fischer Verlag
Rights: fechado
Date Issue: 1995
Appears in Collections:Unicamp - Artigos e Outros Documentos

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