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Type: Artigo de periódico
Title: Exacerbation of Leishmania (Viannia) shawi infection in BALB/c mice after immunization with soluble antigen from amastigote forms
Author: PASSERO, Luiz Felipe Domingues
BORDON, Maria Luiza Antunes da Costa
CARVALHO, Ana Kely de
MARTINS, Luciano Moura
CORBETT, Carlos Eduardo Pereira
LAURENTI, Marcia Dalastra
Abstract: The present study aimed to evaluate the effects of immunization with soluble amastigote (AmaAg) and promastigote (ProAg) antigens from Leishmania (Viannia) shawi on the course of infection in BALB/c mice. After immunization with AmaAg, the challenged group showed greater lesion size and parasite load in the skin and lymph nodes, associated with diminished interleukin (IL)-2, IL-4, IL-10, interferon (IFN)-gamma and nitrate levels in the supernatant of lymph node cell cultures, together with increases in transforming growth factor (TGF)-beta concentrations and humoral immune response. In contrast, immunization with ProAg led to smaller lesion size with reduced numbers of viable parasites in the skin. Protection was associated with increases in IL-12, IFN-gamma, TGF-beta and nitrates and decreases in IL-4 and IL-10 levels. Concerning humoral immune response, a significant reduction in anti-leishmania immunoglobulin G was verified in the ProAg-challenged group. Analysis of these results suggests that AmaAg induced a suppressive cellular immune response in mice, favouring the spread of infection, whereas ProAg induced partial protection associated with increased cellular immune response.
Subject: Leishmania (Viannia) shawi
amastigote antigen
promastigote antigen
Country: Estados Unidos
Citation: APMIS, v.118, n.12, p.973-981, 2010
Rights: fechado
Identifier DOI: 10.1111/j.1600-0463.2010.02679.x
Date Issue: 2010
Appears in Collections:IB - Artigos e Outros Documentos

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