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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleN-RAS and K-RAS gene mutations in Brazilian patients with multiple myelomapt_BR
dc.contributor.authorOrtega, MMpt_BR
dc.contributor.authorFaria, RMDpt_BR
dc.contributor.authorShitara, ESpt_BR
dc.contributor.authorAssis, AMpt_BR
dc.contributor.authorAlbuquerque, DMpt_BR
dc.contributor.authorOliveira, JSRpt_BR
dc.contributor.authorNoguti, MAEpt_BR
dc.contributor.authorFaria, JRpt_BR
dc.contributor.authorCosta, FFpt_BR
dc.contributor.authorLima, CSPpt_BR
unicamp.author.emailcarmenl@fcm.unicamp.brpt_BR
unicamp.authorState Univ Campinas, Dept Internal Med, BR-13083970 Campinas, SP, Brazil Univ Fed Sao Paulo, Paulista Sch Med, Sao Paulo, Brazil State Univ Campinas, Haematol & Hameotherapy Ctr, Sao Paulo, Brazilpt_BR
dc.subjectmultiple myelomapt_BR
dc.subjectmutationpt_BR
dc.subjectN-RASpt_BR
dc.subjectK-RASpt_BR
dc.subject.wosActivating Mutationspt_BR
dc.subject.wosAmplificationpt_BR
dc.subject.wosOncogenespt_BR
dc.subject.wosLeukemiapt_BR
dc.subject.wosBrafpt_BR
dc.subject.wosP53pt_BR
dc.subject.wosDnapt_BR
dc.description.abstractPoint mutations affecting codons 12, 13 (exon 1) and 61 (exon 2) of the N-RAS gene and codons 12 and 13 (exon 1) of the K-RAS gene are identified in approximately 30.0% and 10.0%, respectively, of multiple myeloma (MM) patients living in the northern hemisphere. To date, there are no reports about the prevalence of RAS gene mutations in MM Brazilian patients, and this comprised the aim of the present study. DNA from bone marrow aspirates of 252 patients with MM (139 males and 113 females; aged 59.33 +/- 11.95 years) were investigated for whole exons 1 and 2 of the N-RAS gene and whole exon 1 of the K-RAS gene by direct sequencing of DNA amplified in vitro by the polymerase chain reaction. Fifty-three out of 252 (21.03%) MM patients presented RAS mutations. Heterozygous mutations at codons 4, 10 (exon 1), 61 and 65 (exon 2) of the N - RAS gene were identified in seven out of 252 (2.78%) patients. K-RAS heterozygous mutations at codons 7, 12, 13 (exon 1) were seen in 46 out of 252 (18.25%) patients. To the best of our knowledge, the mutation at codon 7 of K-RAS gene is reported for the first time in MM. Taken together, these results suggest that Brazilian MM patients are characterized by: (i) a low prevalence of RAS mutation and (ii) RAS mutations located at distinct regions of the critical codons of the N - RAS and K-RAS genes.pt
dc.relation.ispartofLeukemia & Lymphomapt_BR
dc.relation.ispartofabbreviationLeuk. Lymphomapt_BR
dc.publisher.cityAbingdonpt_BR
dc.publisher.countryInglaterrapt_BR
dc.publisherTaylor & Francis Ltdpt_BR
dc.date.issued2006pt_BR
dc.date.monthofcirculationFEBpt_BR
dc.identifier.citationLeukemia & Lymphoma. Taylor & Francis Ltd, v. 47, n. 2, n. 285, n. 289, 2006.pt_BR
dc.language.isoenpt_BR
dc.description.volume47pt_BR
dc.description.issuenumber2pt_BR
dc.description.firstpage285pt_BR
dc.description.lastpage289pt_BR
dc.rightsfechadopt_BR
dc.rights.licensehttp://journalauthors.tandf.co.uk/permissions/reusingOwnWork.asppt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn1042-8194pt_BR
dc.identifier.wosidWOS:000233641400012pt_BR
dc.identifier.doi10.1080/10428190500300969pt_BR
dc.date.available2014-11-15T01:28:05Z
dc.date.available2015-11-26T17:18:13Z-
dc.date.accessioned2014-11-15T01:28:05Z
dc.date.accessioned2015-11-26T17:18:13Z-
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dc.description.provenanceMade available in DSpace on 2015-11-26T17:18:13Z (GMT). No. of bitstreams: 2 WOS000233641400012.pdf: 176269 bytes, checksum: ba77a603c66ca7b6b48b0dd149694fa1 (MD5) WOS000233641400012.pdf.txt: 17711 bytes, checksum: c7164611c5a987c366e18aa5f4d00f5e (MD5) Previous issue date: 2006en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/62174pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/62174
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/62174-
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