Please use this identifier to cite or link to this item:
http://repositorio.unicamp.br/jspui/handle/REPOSIP/61959
Type: | Artigo |
Title: | Nonadrenergic, noncholinergic relaxation of human isolated corpus cavernosum induced by scorpion venom |
Title Alternative: | |
Author: | Teixeira, C.E. Moreno, R.A. Faro, R. De Nucci, G. Antunes, E. Netto, N.R. Fregonesi, A. |
Abstract: | To examine the effects of Tityus serrulatus scorpion venom (TSV) on human corpus cavernosum (HCC) using a bioassay cascade. Priapism is occasionally observed in scorpion envenomation, mostly in children. Methods. HCC strips were suspended in a cascade system and superfused with aerated and warmed Krebs' solution at 5 mL/min. Noradrenaline (5 mu mol/L) was infused to induce a submaximal contraction of the HCC strips. The release of cyclooxygenase products was prevented by infusing indomethacin (6 mu mol/L). Results. N-omega-nitro-L-arginine methyl ester (10 mu mol/L; n = 10) increased the tone of the preparations and significantly reduced (P <0.01)the acetylcholine (ACh) and TSV-induced relaxations. Subsequent infusion of L-arginine (300 <mu>mol/L) partially reversed the increased tone and significantly restored the relaxations induced by TSV and ACh (P <0.01). The soluble guanylyl cyclase inhibitor ODQ (10 <mu>mol/L; n = 8) markedly reduced (P <0.01) the relaxations induced by TSV, ACh, glyceryl trinitrate, and bradykinin. 7-Nitroindazole (10 <mu>mol/L; n = 8) inhibited the relaxations induced by TSV by 84% (P <0.01) and also caused small, but significant, reductions in the ACh and bradykinin-induced HCC relaxations (P <0.05). Atropine (1 mu mol/L; n = 6) abolished the relaxations evoked by ACh (P <0.01), but had no effect on those elicited by TSV. Tetrodotoxin (1 <mu>mol/L; n = 6) abolished the relaxations induced by TSV (P <0.01) and also reversed the established TSV-induced relaxation (n = 4). Conclusions, Our results indicate that TSV relaxes HCC through the release of nitric oxide from nonadrenergic, noncholinergic (NANC) nerves. The elucidation of the mechanism responsible for the TSV-induced relaxations might be useful for a better understanding of the development of priapism in cases of scorpion envenomation |
metadata.dc.description.abstractalternative: | |
Subject: | Acetilcolina Arginina Bradicinina Inibidores de ciclo-oxigenase Histamina |
Country: | Estados Unidos |
Editor: | Elsevier |
Citation: | Urology. Elsevier Science Inc, v. 57, n. 4, n. 816, n. 820, 2001. |
Rights: | fechado |
Identifier DOI: | 10.1016/S0090-4295(00)01047-5 |
Address: | https://www.goldjournal.net/article/S0090-4295(00)01047-5/abstract |
Date Issue: | 2001 |
Appears in Collections: | FCM - Artigos e Outros Documentos |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
000168079700065.pdf | 273.46 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.