Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/61797
Type: Artigo de periódico
Title: Differential enumeration of Bifidobacterium longum and Lactobacillus acidophilus in the presence of Streptococcus thermophilus
Author: Zacarchenco, PB
Massaguer-Roig, S
Abstract: Modified (T and M-MRS) and selective (Bile and LP-MRS) media were assayed to quantify Bifidobacterium longum (BL) and Lactobacillus acidophilus (LAC) in the presence of Streptococcus thermophilus (Str). Microbial suspensions (isolated and mixed) were tested at 37degrees/72 h aerobically with T-MRS, M-MRS and Bile-MRS; all tests using LP-MRS were done anaerobically. LP-MRS was tested under 4 different combinations of lithium chloride and sodium propionate concentrations (0.2; 0.4; 0.5 and 0.6% of LiCl with respectively 0.3; 0.6; 0.75 and 0.9% of sodium propionate). The ability of each medium to inhibit the organisms was also tested. M-MRS allowed the growth of Str. Differential counting of LAC was possible using T and Bile-MRS, aerobically, (the latter condition being more practical and economical). Differential counting of BL was possible using LP-MRS in anaerobiosis, with a combination of 0.6% of LiCl and 0.9% of sodium propionate.
Country: Alemanha
Editor: A V A Agrarverlag
Rights: fechado
Date Issue: 2004
Appears in Collections:Unicamp - Artigos e Outros Documentos

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