Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/61741
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampGoncalves, Letícia Machadopt_BR
dc.contributor.authorunicampVasconcellos, Andréa Araújo dept_BR
dc.contributor.authorunicampSilva, Wander José dapt_BR
dc.contributor.authorunicampCury, Jaime Aparecidopt_BR
dc.contributor.authorunicampDel Bel Cury, Altair Antoninhapt_BR
dc.typeArtigopt_BR
dc.titleDietary carbohydrates modulate Candida albicans biofilm development on the denture surfacept_BR
dc.contributor.authorSantana, I. L.pt_BR
dc.contributor.authorGoncalves, L. M.pt_BR
dc.contributor.authorde Vasconcellos, A. A.pt_BR
dc.contributor.authorda Silva, W. J.pt_BR
dc.contributor.authorCury, J. A.pt_BR
dc.contributor.authorCury, A. A. D.pt_BR
dc.subjectBiofilmespt_BR
dc.subjectCandida Albicanspt_BR
dc.subjectCarboidratos da dietapt_BR
dc.subject.otherlanguageDietary carbohydratespt_BR
dc.subject.otherlanguageCandida Albicanspt_BR
dc.subject.otherlanguageBiofilmspt_BR
dc.description.abstractThe purpose of this study was to investigate whether dietary carbohydrates can modulate the development of Candida albicans biofilms on the denture material surface. Poly (methyl methacrylate) acrylic resin discs were fabricated and had their surface roughness measured. Biofilms of C. albicans ATCC 90028 were developed on saliva-coated specimens in culture medium without (control) or with carbohydrate supplementation by starch, starch+sucrose, glucose, or sucrose for 72 h. The cell count, metabolic activity, biovolume, average thickness, and roughness coefficient were evaluated at the adhesion phase (1.5 h) and after 24, 48, and 72 h. The secretion of proteinases and phospholipases, cell surface energy, and production of extra/intracellular polysaccharides were analyzed after 72 h of biofilm development. Data were analyzed by one-and two-way ANOVA followed by Tukey's test at 5% significance level. In the early stages of colonization (adhesion and 24 h), the glucose group showed the highest cell counts and metabolic activity among the groups (p < 0.05). After maturation (48 and 72 h), biofilms exposed to glucose, sucrose, or starch+sucrose showed higher cell counts and metabolic activity than the control and starch groups (p < 0.001). Compared to the control group, biofilms developed on starch or starch+sucrose had more proteinase activity (p < 0.001), whereas biofilms developed on glucose or sucrose had more phospholipase activity (p < 0.05). Exposure to starch+sucrose increased the production of extracellular and intracellular polysaccharides (p < 0.05). Biofilms developed on starch or without carbohydrate supplementation presented cells with more hydrophobic behavior compared to the other groups. Confocal images showed hyphae forms on biofilms exposed to starch or starch+sucrose. Within the conditions studied, it can be concluded that dietary carbohydrates can modulate biofilm development on the denture surface by affecting virulence factors and structural featurespt
dc.relation.ispartofPLoS onept_BR
dc.relation.ispartofabbreviationPLoS onept_BR
dc.publisher.citySan Francisco, CApt_BR
dc.publisher.countryEstados Unidospt_BR
dc.publisherPublic Library of Sciencept_BR
dc.date.issued2013pt_BR
dc.date.monthofcirculationMaypt_BR
dc.identifier.citationPlos One. Public Library Science, v. 8, n. 5, 2013.pt_BR
dc.language.isoengpt_BR
dc.description.volume8pt_BR
dc.description.issuenumber5pt_BR
dc.description.firstpage1pt_BR
dc.description.lastpage7pt_BR
dc.rightsabertopt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.eissn1932-6203pt_BR
dc.identifier.doi10.1371/journal.pone.0064645pt_BR
dc.identifier.urlhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0064645pt_BR
dc.description.sponsorshipCONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQpt_BR
dc.description.sponsorship1CNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOpt_BR
dc.description.sponsordocumentnumberBrazilian National Agency for Science and Technology [2006-7/308141]pt
dc.description.sponsordocumentnumber2006-7/308141pt_BR
dc.date.available2014-07-30T14:42:26Z
dc.date.available2015-11-26T17:39:11Z-
dc.date.accessioned2014-07-30T14:42:26Z
dc.date.accessioned2015-11-26T17:39:11Z-
dc.description.provenanceMade available in DSpace on 2014-07-30T14:42:26Z (GMT). No. of bitstreams: 0 Previous issue date: 2013. Added 1 bitstream(s) on 2019-02-26T18:46:44Z : No. of bitstreams: 1 000321394700056.pdf: 691371 bytes, checksum: d42cc58dd86e7da4ad9b912de793157c (MD5) Bitstreams deleted on 2020-05-14T20:38:32Z: 000321394700056.pdf,. Added 1 bitstream(s) on 2020-05-14T20:42:46Z : No. of bitstreams: 1 000321394700056.pdf: 483347 bytes, checksum: 80fc0b1a99abf8edffd9f52dd90fdc47 (MD5) Bitstreams deleted on 2020-06-04T12:47:36Z: 000321394700056.pdf,. Added 1 bitstream(s) on 2020-06-04T12:52:45Z : No. of bitstreams: 1 000321394700056.pdf: 1181240 bytes, checksum: 9de20dfe1d10ed7eda077ffc7306cc87 (MD5)en
dc.description.provenanceMade available in DSpace on 2015-11-26T17:39:11Z (GMT). No. of bitstreams: 0 Previous issue date: 2013en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/61741
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/61741-
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentDepartamento de Prótese e Periodontiapt_BR
dc.contributor.departmentDepartamento de Ciências Fisiológicaspt_BR
dc.contributor.departmentDepartamento de Prótese e Periodontiapt_BR
dc.contributor.unidadeFaculdade de Odontologia de Piracicabapt_BR
dc.identifier.source000321394700056-
dc.creator.orcidsem informaçãopt_BR
dc.creator.orcidsem informaçãopt_BR
dc.creator.orcid0000-0002-5613-5081pt_BR
dc.creator.orcid0000-0003-1046-5605pt_BR
dc.creator.orcid0000-0002-4329-0437pt_BR
dc.type.formArtigopt_BR
dc.identifier.articleide64645pt_BR
dc.identifier.articleide64645-
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