Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/61638
Type: Artigo de periódico
Title: Development, characterization, and comparative analysis of polymorphism at common bean SSR loci isolated from genic and genomic sources
Author: Hanai, LR
de Campos, T
Camargo, LEA
Benchimol, LL
de Souza, AP
Melotto, M
Carbonell, SAM
Chioratto, AF
Consoli, L
Formighieri, EF
Siqueira, MVBM
Tsai, SM
Vieira, MLC
Abstract: Microsatellites or SSRs (single sequence repeats) have been used to construct and integrate genetic maps in crop species, including Phaseolus vulgaris. ln the present study, 3 cDNA libraries generated by the Bean EST project (http://1gm.esalq.usp.br/BEST/), comprising a unigene collection of 3126 sequences and a genomic microsatellite-enriched library, were analyzed for the presence of SSRs. A total of 219 expressed sequence tags (ESTs) were found to carry 240 SSRs (named EST-SSR), whereas 714 genomic sequences contained 471 SSRs (named genomic-SSR). A subset of 80 SSRs, 40 EST-SSRs, and 40 genomic-SSRs were evaluated for molecular polymorphism in 23 genotypes of cultivated beans from the Mesoamerican and Andean genetic pools, including Brazilian cultivars and 2 related species. Of the common bean genotypes, 31 EST-SSR loci were polymorphic, yielding 2-12 alleles as compared with 26 polymorphic genomic-SSRs, accounting for 2-7 alleles. Cluster analysis from data using both genic and genomic-SSR revealed a clear separation between Andean and Mesoamerican beans. The usefulness of these loci for distinguishing bean genotypes and genetic mapping is discussed.
Subject: Phaseolus vulgaris
microsatellites
genetic markers
expressed sequence tags (EST)
Country: Canadá
Editor: Natl Research Council Canada-n R C Research Press
Rights: fechado
Identifier DOI: 10.1139/G07-007
Date Issue: 2007
Appears in Collections:Unicamp - Artigos e Outros Documentos

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