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Type: Artigo de periódico
Title: Development of an enzymeless biosensor for the determination of phenolic compounds
Author: Sotomayor, MDT
Tanaka, AA
Kubota, LT
Abstract: The construction of amperometric enzymeless biosensors for phenolic compounds determination, using carbon paste electrode modified with copper phtalocyanine (CuPc) and histidine (His), based on the chemistry of the dopamine beta-monooxygenase (DbetaM) enzyme that catalyzes the hydroxylation of the dopamine and its analogs is shown. The modified carbon paste was evaluated on electrodes constructed in two ways: putting the paste into a cavity of a rotating disk electrode and a platinum slide electrode fixed into a glass tube. The sensor in hydrodynamic conditions presented a linear response range between 30 and 250 mumol l(-1), with a sensitivity of 4.6 +/- 0.1 nAl mumol(-1) cm(-2) for catechol, response time of 3 s and lifetime of about 50 days when stored at room temperature. The sensor in static conditions showed a linear response range from 40 to 250 mumol l(-1), with a sensitivity of 0.30 +/- 0.01 nAl mumol(-1) cm(-2) for catechol. The sensors presented the following relative response order for dopamine and some analog species: catechol > dopamine > guaiacol > serotonin > phenol. (C) 2002 Elsevier Science B.V. All rights reserved.
Subject: enzymeless biosensor
copper phtalocyanine
phenolic compounds
Country: Holanda
Editor: Elsevier Science Bv
Rights: fechado
Date Issue: 2002
Appears in Collections:Unicamp - Artigos e Outros Documentos

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