Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/61549
Type: Artigo de periódico
Title: Development of a laccase-based flow injection electrochemical biosensor for the determination of phenolic compounds and its application for monitoring remediation of Kraft E1 paper mill effluent
Author: Freire, RS
Duran, N
Kubota, LT
Abstract: This paper describes the development of a new system for amperometric determination of phenolic compounds, and its application for monitoring these compounds in paper mill effluent. The method was based on a flow system, a dialysis sampler, and a laccase-based biosensor. The performance of this system was investigated with respect to pH, ionic strength, working potential, and flow-rate dependence. The biosensor showed an excellent long-term stability allowing measurements for over than 3 months. The sensitivity of laccase-based biosensor was tested for phenol, p-chlorophenol, guaiacol and chloroguaiacol; the detector presented selective measurements of micromolar concentration of these compounds. The integration of a dialysis membrane sampling in the system protected the biosensor surface from fouling and gave independence of sample conditions that commonly influence the biosensor performance. These favorable characteristics allowed its application for direct measurements in complex media with no sample pretreatment. This ability was continued employing this system in a continuous analysis of phenolic compounds during the remediation of paper mill effluent by ozonization process. (C) 2002 Elsevier Science B.V. All rights reserved.
Subject: laccase
biosensor
environmental monitoring
paper mill effluents
remediatiom
ozonization
Country: Holanda
Editor: Elsevier Science Bv
Rights: fechado
Identifier DOI: 10.1016/S0003-2670(02)00417-8
Date Issue: 2002
Appears in Collections:Unicamp - Artigos e Outros Documentos

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