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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleDevelopment and Validation of a Liquid Chromatography-UV Detection Method for the Determination of Sulfonamides in Fish Muscle and Shrimp According to European Union Decision 2002/657/ECpt_BR
dc.contributor.authorGranja, RHMMpt_BR
dc.contributor.authorde Lima, ACpt_BR
dc.contributor.authorSalerno, AGpt_BR
dc.contributor.authorWanschel, ACBApt_BR
unicamp.author.emailrodrigo@microbioticos.compt_BR
unicamp.authorGranja, Rodrigo H. M. M. de Lima, Andreia C. Salerno, Alessandro G. Microbiot Labs, BR-13084471 Campinas, SP, Brazilpt_BR
unicamp.authorSalerno, Alessandro G. Wanschel, Amarylis C. B. A. Univ Estadual Campinas, Dept Physiol & Biophys, BR-13083970 Campinas, SP, Brazilpt_BR
dc.subject.wosPostcolumn Derivatizationpt_BR
dc.subject.wosFluorescence Detectionpt_BR
dc.subject.wosMass-spectrometrypt_BR
dc.subject.wosAgentspt_BR
dc.description.abstractSulfonamides are one class of antimicrobial agents used in aquaculture production. Sulfonamides are often overused because they are inexpensive and readily available. Their presence at a concentration above the legal limits is a potential hazard to human health. Brazilian authorities have included in the National Regulatory Monitoring Program the control of the three most widely used sulfonamides in aquaculture production, i.e., sulfathiazole, sulfamethazine, and sulfadimethoxine. An LC method with UV detection for the determination of residual sulfonamides in fish muscle, using sulfapyridine as an internal standard has been developed and validated. The validation was performed according to the Brazilian Regulation 2412009 (equivalent to European Union Decision 2002/657/EC). The method meets the Brazilian regulatory requirement that establishes criteria and procedures for determination of parameters such as decision limit (CC alpha), detection capability (CC beta), precision, and recovery. For fish muscle, CC alpha was determined at 3.63, 2.91, and 7.46 mu g/kg for sulfathiazole, sulfamethazine, and sulfadimethoxine, respectively. CC beta was 9.39, 14.54, and 9.39 mu g/kg for sulfathiazole, sulfamethazine, and sulfadimethoxine, respectively. For shrimp, CC alpha was 11.5, 8.67, and 4.46 mu g/kg for sulfathiazole, sulfamethazine, and sulfadimethoxine, respectively. CC beta was 18, 11.93, and 5.24 mu g/kg for sulfathiazole, sulfamethazine, and sulfadimethoxine, respectively. A complete statistical analysis was performed on the results obtained. The results indicate that the method is robust when subjected to day-to-day analytical variations.pt
dc.relation.ispartofJournal Of Aoac Internationalpt_BR
dc.relation.ispartofabbreviationJ. AOAC Int.pt_BR
dc.publisher.cityGaithersburgpt_BR
dc.publisher.countryEUApt_BR
dc.publisherAoac Intpt_BR
dc.date.issued2013pt_BR
dc.date.monthofcirculationJAN-FEBpt_BR
dc.identifier.citationJournal Of Aoac International. Aoac Int, v. 96, n. 1, n. 212, n. 215, 2013.pt_BR
dc.language.isoenpt_BR
dc.description.volume96pt_BR
dc.description.issuenumber1pt_BR
dc.description.firstpage212pt_BR
dc.description.lastpage215pt_BR
dc.rightsfechadopt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn1060-3271pt_BR
dc.identifier.wosidWOS:000315254300032pt_BR
dc.identifier.doi10.5740/jaoacint.11-541pt_BR
dc.date.available2014-07-30T14:39:50Z
dc.date.available2015-11-26T17:37:51Z-
dc.date.accessioned2014-07-30T14:39:50Z
dc.date.accessioned2015-11-26T17:37:51Z-
dc.description.provenanceMade available in DSpace on 2014-07-30T14:39:50Z (GMT). No. of bitstreams: 0 Previous issue date: 2013en
dc.description.provenanceMade available in DSpace on 2015-11-26T17:37:51Z (GMT). No. of bitstreams: 0 Previous issue date: 2013en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/61530
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/61530-
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