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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleDetection of hematopoietic maturation abnormalities by flow cytometry in myelodysplastic syndromes and its utility for the differential diagnosis with non-clonal disorderspt_BR
dc.contributor.authorLorand-Metze, Ipt_BR
dc.contributor.authorRibeiro, Ept_BR
dc.contributor.authorLima, CSPpt_BR
dc.contributor.authorBatista, LSpt_BR
dc.contributor.authorMetze, Kpt_BR
unicamp.author.emaililmetze@unicamp.brpt_BR
unicamp.authorUniv Estadual Campinas, Dept Internal Med, Hemctr, BR-13081970 Campinas, SP, Brazil Univ Salamanca, Ctr Invest Canc, Serv Gen Citometria, Dept Med, E-37008 Salamanca, Spain Univ Estadual Campinas, Dept Pathol, Sao Paulo, Brazilpt_BR
dc.subjectmyelodysplastic syndromes bone marrowpt_BR
dc.subjectcell maturationpt_BR
dc.subjectantigen expressionpt_BR
dc.subjectmonocytespt_BR
dc.subjectflow cytometrypt_BR
dc.subject.wosBone-marrowpt_BR
dc.subject.wosClassificationpt_BR
dc.subject.wosExpressionpt_BR
dc.subject.wosDysplasiapt_BR
dc.subject.wosMonocytespt_BR
dc.subject.wosLeukemiaspt_BR
dc.subject.wosAntigenspt_BR
dc.subject.wosSurvivalpt_BR
dc.subject.wosFeaturespt_BR
dc.subject.wosAnemiapt_BR
dc.description.abstractThe diagnosis of myelodysplastic syndromes (MDS) is based on peripheral cytopenias, bone marrow (BM) morphology and karyotyping. This may be difficult in cases with few dysplastic elements in BM and a normal karyotype. We examined the utility of flow cytometric analysis for the differential diagnosis between MDS and non-clonal disorders (NCD) presenting peripheral cytopenias. Quantitative assessment of CD45. CD16. CD13. CD11b, CD10 and CD64 in granulocytes and monocytes, and CD71 and glycophorin A in erythroblasts besides CD34+ cell count was performed in BM of 31 consecutive newly diagnosed patients with MDS, I I patients with NCD and I I healthy controls (BM donors). In MDS, the median number of phenotypic abnormalities found was 3 (1-8). The WPSS score showed a correlation with the total number of changes per case (r=0.48; p=0.002). Decreased SSC in promyelocytes correlated with the peripheral neutrophil count (r = -0.46; p = 0.007). In NCD, the normal variation of antigen expression along granulocytic and erythroblast maturation was always maintained. In the discriminant analysis, SSC of CD34+ cells, together with that of promyelocytes and metamyelocytes were able to correctly classify 87% of the cases as clonal or non-clonal. Our quantitative approach permitted to detect at least one abnormality in antigen expression in every case of MDS. However. the most important parameters for differential diagnosis with NCD were the analysis of the granularity in immature cells, especially of the granulocytic series. (c) 2006 Elsevier Ltd. All rights reserved.pt
dc.relation.ispartofLeukemia Researchpt_BR
dc.relation.ispartofabbreviationLeuk. Res.pt_BR
dc.publisher.cityOxfordpt_BR
dc.publisher.countryInglaterrapt_BR
dc.publisherPergamon-elsevier Science Ltdpt_BR
dc.date.issued2007pt_BR
dc.date.monthofcirculationFEBpt_BR
dc.identifier.citationLeukemia Research. Pergamon-elsevier Science Ltd, v. 31, n. 2, n. 147, n. 155, 2007.pt_BR
dc.language.isoenpt_BR
dc.description.volume31pt_BR
dc.description.issuenumber2pt_BR
dc.description.firstpage147pt_BR
dc.description.lastpage155pt_BR
dc.rightsfechadopt_BR
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policypt_BR
dc.sourceWeb of Sciencept_BR
dc.identifier.issn0145-2126pt_BR
dc.identifier.wosidWOS:000243631300005pt_BR
dc.identifier.doi10.1016/j.leukres.2006.04.010pt_BR
dc.date.available2014-11-16T16:48:37Z
dc.date.available2015-11-26T16:23:59Z-
dc.date.accessioned2014-11-16T16:48:37Z
dc.date.accessioned2015-11-26T16:23:59Z-
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dc.description.provenanceMade available in DSpace on 2015-11-26T16:23:59Z (GMT). No. of bitstreams: 2 WOS000243631300005.pdf: 1002554 bytes, checksum: 7c068992678d0a2b28de63f9aad75b50 (MD5) WOS000243631300005.pdf.txt: 33549 bytes, checksum: aecb02c8263d9d35daf8900aabbbf564 (MD5) Previous issue date: 2007en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/61308pt_BR
dc.identifier.urihttp://www.repositorio.unicamp.br/handle/REPOSIP/61308
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/61308-
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