Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/61292
Type: Artigo de periódico
Title: Detection of bovine respiratory syncytial virus in experimentally infected balb/c mice
Author: Almeida, RS
Domingues, HG
Coswig, LT
D'arce, RCF
de Carvalho, RF
Arns, CW
Abstract: The present study used an RT-nested-PCR and an immunohistochemistry assay to detect bovine respiratory syncytial virus in tissues from experimentally infected balb/c mice. As a first step, Chicken Embryo Related (CER) cell monolayers infected with the BRSV-25-BR strain isolated in Brazil were used for antigen production. Then, the infected lung and tracheal tissues of female balb/c mice were collected on 3, 5, 7 and 10 days post-infection and submitted to both techniques. Primers specific to F and G genes that amplify fragments of 481 bp and 371 bp, respectively, were used. The BRSV detection was not successful in all of the animals tested. The genomic fragment of the G gene from the organs of some infected mice on all analyzed post-infection days was amplified. However, in the RT-nested-PCR corresponding to the F gene, it was not possible to observe any amplified fragment. This was probably due to the higher sensitivity of the developed technique to amplify the fragment corresponding to the G gene compared to the F gene. Moreover, only three of the lungs collected five days post-infection were positive by immunohistochemistry. To the author's knowledge, this is the first study reporting bovine respiratory syncytial virus detection in balb/c mice after experimental inoculation.
Subject: bovine respiratory syncytial virus
RT-nested-PCR
immunohistochemistry
mice
experimental infection
Country: França
Editor: E D P Sciences
Rights: aberto
Identifier DOI: 10.1051/vetres:2004003
Date Issue: 2004
Appears in Collections:Unicamp - Artigos e Outros Documentos

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