Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/61291
Type: Artigo de periódico
Title: DETECTION OF BORRELIA-BURGDORFERI IN BIOLOGICAL SAMPLES USING THE POLYMERASE CHAIN-REACTION ASSAY
Author: DEBUE, M
GAUTIER, P
HACKEL, C
VANELSEN, A
HERZOG, A
BIGAIGNON, G
BOLLEN, A
Abstract: Oligonucleotide primers were used in the polymerase chain reaction assay to amplify specific DNA regions of the Borrelia burgdorferi 49-kb linear plasmid. One set of primers identifies a 442-bp DNA fragment in the OspA gene and a second pair of amplimers, a 176-bp DNA piece located in the OspB gene. The last set of primers, OspBpc3/pc4, outperformed the other pair in discriminating pathogenic North American or European isolates from related bacterial species, detected down to 4 spirochaetes, and was suitable for the identification of B. burgdorferi in biological samples, such as synovial and cerebrospinal fluids.
Subject: BORRELIA-BURGDORFERI
LYME DISEASE
PCR
DIAGNOSIS
CEREBROSPINAL AND SYNOVIAL FLUIDS
Country: França
Editor: Editions Scientifiques Elsevier
Rights: fechado
Identifier DOI: 10.1016/0923-2508(91)90189-H
Date Issue: 1991
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File Description SizeFormat 
WOSA1991FZ02100008.pdf1.1 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.