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Type: Artigo de periódico
Title: Kinetic method for the determination of alpha-methyldopa in pharmaceutical preparations: Analytical procedure and reaction mechanism considerations
Author: Tubino, M
Batista, DCDV
Rodrigues, JAR
Abstract: A reliable and very simple kinetic method is proposed for the determination of a-methyldopa in pharmaceutical preparations. It is based of the oxidation of a-methyldopa, a catechol derivative, to quinone, by the ferric ion in the presence of salicylic acid and HCl. The deep blue complex formed between iron(III) and salicylate (lambda(max) 525 nm) allows the reaction to be watched as absorbance decreases with the reduction of the ferric ions to ferrous with the consequent dissociation of the complex. Four pharmaceutical preparations were analyzed and the results were compared with those obtained with the spectrophotometric United States Pharmacopeia method. The statistical t-Student and the F-tests were applied to compare the results obtained with the two independent methods. In all cases complete agreement, in terms of accuracy and precision, was observed with a confidence level of 95% (alpha=0.05). Considering the four samples analyzed, using the proposed method, and five determinations for each sample, the observed mean relative standard deviation (RSD) is about 0.8%. The concentration range studied was from about 2 x 10(-4) mol L-1 to 18 x 10(-4) mol L-1 (about 40 mu g/mL to 360 mu g/mL) in the final solution and it is quite adequate for the analytical procedure at 25.0 +/- 0.1 degrees C. A typical coefficient of determination, r(2) , of the calibration curve, is 0.9994. For the kinetic pseudo first order curves a typical observed r(2) is 0.99998. The Arrhenius activation energy was found to be 84.2 +/- 3.4 kJ mol(-1) . A schematic mechanism of the reaction is proposed.
Subject: alpha-methyldopa
kinetic method
pharmaceutical preparations
Country: EUA
Editor: Taylor & Francis Inc
Rights: fechado
Identifier DOI: 10.1080/00032710500477050
Date Issue: 2006
Appears in Collections:Unicamp - Artigos e Outros Documentos

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