Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/60927
Type: Artigo de periódico
Title: KARYOTYPE AND NUCLEOLUS ORGANIZER REGIONS OF Ophisternon aenigmaticum (Teleostei: Synbranchiformes: Synbranchidae) FROM VENEZUELA
Author: Nirchio, M
Mariguela, TC
Ferreira, IA
Foresti, F
Oliveira, C
Abstract: The first cytogenetic analysis by conventional Giemsa staining silver nitrate impregnation of nucleolus organizer regions (Ag-NOR), and fluorescence in situ hybridization (FISH) carried out on Ophisternon aenigmaticum from Margarita Island, Venezuela, is reported. Two cytotypes were found: cytotype C1, 2n=46, composed of 6 medium size metacentrics, 2 subtelocentrics and 38 acrocentrics; and cytotype C2, 2n=45, composed of one large metacentric plus 5 medium size metacentrics, I subtelocentric and 38 acrocentric chromosomes. In cytotype C1, major ribosomal DNA (rDNA) cistrons are located in the terminal position of the short arms of the first metacentric pair as determined by Ag-NORs and FISH with the 18S rDNA probe. In cytotype C2, silver staining revealed only one positive signal in a single chromosome of the pair 1 whereas FISH with the 18S rDNA probe hybridized in two different chromosomes: one coincident with the Ag-NOR (pair 1) and other in one acrocentric chromosome, suggesting the occurrence of a pen-centric inversion. In both cytotypes, FISH with 5S rDNA probe produced hybridization signals in subtelomeric position of the short arm of the chromosome pair N(o) 3. Chromosome evolution in Synbranchidae is discussed.
Subject: Chromosome Evolution
Chromosome Fusion
18S DNA
Fish Chromosome
Pericentric Inversion
5S rDNA
Country: Venezuela
Editor: Interciencia
Rights: aberto
Date Issue: 2011
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File Description SizeFormat 
WOS000289125600011.pdf472.11 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.