Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/60853
Type: Artigo de periódico
Title: ISOLATION OF THE LECTIN AND AN L(4) ISOLECTIN FROM PHASEOLUS-VULGARIS BY AFFINITY-CHROMATOGRAPHY ON INSOLUBLE OVOMUCOID
Author: GONCALVES, RB
COSTA, GP
Abstract: Lectins from extracts of Phaseolus vulgaris seeds have potent cell-agglutinating and lymphocyte-stimulating activity. An affinity adsorbent for lectins with specificity for the oligosaccharide structure was prepared by transforming ovomucoid, an oligosaccharide-rich glycoprotein, into an insoluble and stable gel. The ovomucoid was made insoluble by boiling a 20% solution (200 mg/ml) in 0.1 M Tris-HCl, pH 8.9, for 20 min. This insoluble gel was desialylated by treatment with 50 mN sulfuric acid for 1 h at 90 degrees C and fixed with 1% glutaraldehyde, pH 7.4, for 10 min. The Phaseolus lectin and the L(4). isolectin could be isolated essentially in a single-step procedure, using different eluting conditions: 50 mM sodium formate buffer, pH 3.0, was used for PHA elution; a different column was eluted with 15 mM sodium tetraborate, pH 8.0, for desorbed Lq isolectin. Polyacrylamide gel electrophoresis of the lectin showed five distinct bands, whereas the L(4) isolectin only presented one band. From 250 mg of saturated column, 8.25 mg of PHA was isolated. This adsorbent could be used several times with little change in binding capacity or selectivity.
Subject: LECTIN
ISOLECTIN
PHASEOLUS VULGARIS
LECTIN AFFINITY
CHROMATOGRAPHY
Country: Brasil
Editor: Assoc Bras Divulg Cientifica
Rights: aberto
Date Issue: 1995
Appears in Collections:Unicamp - Artigos e Outros Documentos

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