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Type: Artigo
Title: Isolation of a polypeptide from phoneutria nigriventer spider venom responsible for the increased vascular permeability in rabbit skin
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Author: Mancuso, L. C.
Giglio, J.R.
Bento, A. C.
Marangoni, S.
Rego, E.
Novello, J.C.
De Nucci, G.
Oliveira, B.
Antunes, E.
Pedroso-Mariani, S.R.
Caracelli, I.
Abstract: Fractionation of Phoneutria nigriventer venom by Sephadex G-10 followed by ion-exchange chromatography yields a fraction (fraction XIII) which increases microvascular permeability in rabbit skin in vivo by activating the tissue kallikrein-kinin system. One polypeptide (PNV3) with the ability to increase microvascular permeability in the rabbit skin in vivo was isolated from fraction XIII and biochemically characterized. PNV3 has 132 amino acid residues with a calculated mel. wt of 14,475. This polypeptide showed the following N-terminal sequence: AVFAIQDQPC. Amino acid analysis indicated the presence of six disulfide bridges and a high content of Glx (20%). Pairwise comparison of PNV3 amino acid sequence with 27 other spider venom polypeptides and proteins indicated that PNV3 presents high similarity (60-70%) with other toxins (Tx2.1, Tx2.5 and Tx2.6) isolated from P. nigriventer venom
Subject: Sequência de aminoácidos
Peptídeo hidrolases
Venenos de aranha
Country: Reino Unido
Editor: Elsevier
Citation: Toxicon. Pergamon-elsevier Science Ltd, v. 33, n. 2, n. 171, n. 178, 1995.
Rights: fechado
Identifier DOI: 10.1016/0041-0101(94)00148-2
Date Issue: 1995
Appears in Collections:IB - Artigos e Outros Documentos
FCM - Artigos e Outros Documentos

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