Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/60826
Type: Artigo de periódico
Title: Isolation and characterization of novel neurotoxins from south American rattlesnake Crotalus durissus terrificus: Optimization of chromatographic procedure
Author: Toyama, MH
Leite, GB
Rodrigues-Simioni, L
Hernandez, OSS
Novello, JC
Marangoni, S
Abstract: Molecular exclusion HPLC chromatography on a Protein Pak SW 300 column and reversed phase HPLC on a mu -Bondapack C18 column were used to purify four novel neurotoxins from the crotoxin complex, and five crotoxin subunits (PLA(2) and crotapotin) as well as two crotamine isoforms. A single preparative reversed phase HPLC steps yielded a major basic myotoxin followed by the subunits of crotoxin (three PLA(2) isoforms and two crotapotin isoforms). This modified procedure may be useful for the rapid, large-scale purification of toxins from Crotalid venoms.
Subject: reversed phase HPLC
molecular exclusion
snake venom
Crotalus durissus terrificus
rattlesnake
Country: Holanda
Editor: Bentham Science Publ Ltd
Rights: fechado
Date Issue: 2000
Appears in Collections:Unicamp - Artigos e Outros Documentos

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