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|Type:||Artigo de periódico|
|Title:||Isolation and characterization of novel neurotoxins from south American rattlesnake Crotalus durissus terrificus: Optimization of chromatographic procedure|
|Abstract:||Molecular exclusion HPLC chromatography on a Protein Pak SW 300 column and reversed phase HPLC on a mu -Bondapack C18 column were used to purify four novel neurotoxins from the crotoxin complex, and five crotoxin subunits (PLA(2) and crotapotin) as well as two crotamine isoforms. A single preparative reversed phase HPLC steps yielded a major basic myotoxin followed by the subunits of crotoxin (three PLA(2) isoforms and two crotapotin isoforms). This modified procedure may be useful for the rapid, large-scale purification of toxins from Crotalid venoms.|
|Subject:||reversed phase HPLC|
Crotalus durissus terrificus
|Editor:||Bentham Science Publ Ltd|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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