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Type: Artigo de periódico
Title: Isolation and characterization of isolectins from Talisia esculenta seeds
Author: Freire, MDM
Machado, OLT
Smolka, MB
Marangoni, S
Novello, JC
Macedo, MLR
Abstract: Four isolectins (TEL-I, TEL-II, TEL-III and TEL-IV ) were isolated from seeds of Talisia esculenta by reverse-phase high-performance liquid chromatography. RP-HPLC was performed on a u-Bondapack C18 column (0.78 cm x 30 cm) (Waters 991-PDA system) at room temperature. Rechromatography of the four fractions on a C18 column under the same conditions yielded lectins with two dissimilar subunits (M-r 20 kDa and 40 kDa) bound noncovalently. The isolectins showed very similar characteristics, such as molecular masses, N-terminal sequences, and hemagglutinating activity, but differed in their isoelectric points and in inhibition by carbohydrates.
Subject: isolectin
reverse-phase HPLC
Talisia esculenta seeds
Country: EUA
Editor: Kluwer Academic/plenum Publ
Rights: fechado
Identifier DOI: 10.1023/A:1012510614429
Date Issue: 2001
Appears in Collections:Unicamp - Artigos e Outros Documentos

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