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Type: Artigo de periódico
Title: Isolation and characterization of enzymes involved in lysine catabolism from sorghum seeds
Author: Fornazier, RF
Gaziola, SA
Helm, CV
Lea, PJ
Azevedo, RA
Abstract: Lysine is an essential amino acid synthesized in plants via the aspartic acid pathway. The catabolism of lysine is performed by the action of two consecutive enzymes, lysine 2-oxoglutarate reductase (LOR, EC and saccharopine dehydrogenase (SDH, EC The final soluble lysine concentration in cereal seeds is controlled by both synthesis and catabolism rates. The production and characterization of high-lysine plants species depends on knowledge of the regulatory aspects of lysine metabolism and manipulation of the key enzymes. We have for the first time isolated, partially purified, and characterized LOR and SDH from developing sorghum seeds, which exhibited low levels of activity. LOR and SDH were only located in the endosperm and were very unstable during the isolation and purification procedures. LOR and SDH exhibited some distinct properties when compared to the enzymes isolated from other plant species, including a low salt concentration required to elute the enzymes during anion-exchange chromatography and the presence of multimeric forms with distinct molecular masses.
Subject: aspartate kinase
lysine 2-oxoglutarate reductase
saccharopine dehydrogenase
Country: EUA
Editor: Amer Chemical Soc
Citation: Journal Of Agricultural And Food Chemistry. Amer Chemical Soc, v. 53, n. 5, n. 1791, n. 1798, 2005.
Rights: fechado
Identifier DOI: 10.1021/jf048525o
Date Issue: 2005
Appears in Collections:Unicamp - Artigos e Outros Documentos

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