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Type: Artigo de periódico
Title: IS6110 restriction fragment length polymorphism of Mycobacterium tuberculosis isolated from patients with pulmonary tuberculosis in Campinas, Brazil. Evidence of intercontinental distribution of strains
Author: Calusni, ALR
Roscani, GN
Villares, MCB
Soini, H
Graviss, EA
Ramos, MD
Abstract: Tuberculosis (TB) is a major concern in developing countries. In Brazil, few genotyping studies have been conducted to verify the number of IS6110 copies present in local prevalent strains of Mycobacterium tuberculosis; the distribution and clustering of strains. IS6110 DNA fingerprinting was performed on a sample of M. tuberculosis isolates from patients with AFB smear-positive pulmonary TB, at a hospital in Brazil. The IS6110 profiles were analyzed and compared to a M. tuberculosis database of the Houston Tuberculosis Initiative, Houston, US. Seventy-six fingerprints were obtained from 98 patients. All M. tuberculosis strains had an IS6110 copy number between 5-21 allowing for differentiation of the isolates. Human immunodeficiency virus infection was confirmed in nearly half the patients of whom data was available. Fifty-eight strains had unique patterns, while 17 strains were grouped in 7 clusters (2 to 6 strains). When compared to the HTI database, 6 strains matched isolates front El Paso, Ciudad de Juarez, Houston, and New York. Recently acquired infections were documented in 19% of cases. The community transmission of infection is intense, since some clustered strains were recovered during the four-year study period. The intercontinental dissemination of M. tuberculosis strains is suspected by demonstration of identical fingerprints in a distant country.
Subject: tuberculosis
Mycobacterium tuberculosis
IS6110 restriction fragment length polymorphism
Country: Brasil
Editor: Fundaco Oswaldo Cruz
Rights: aberto
Identifier DOI: 10.1590/S0074-02762003000500012
Date Issue: 2003
Appears in Collections:Unicamp - Artigos e Outros Documentos

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