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Type: Artigo de periódico
Title: Investigation of red blood cell antigens with highly fluorescent and stable semiconductor quantum dots
Author: de Farias, PMA
Santos, BS
de Menezes, FD
Ferreira, RD
Barias-Castro, ML
Castro, V
Lima, PRM
Fontes, A
Cesar, CL
Abstract: We report a new methodology for red blood cell antigen expression determination by a simple labeling procedure employing luminescent semiconductor quantum dots. Highly luminescent and stable core shell cadmium sulfide/cadmium hydroxide colloidal particles are obtained, with a predominant size of 9 nm. The core-shell quantum dots are functionalized with glutaraldehyde and conjugated to a monoclonal anti-A antibody to target antigen-A in red blood cell membranes. Erythrocyte samples of blood groups A(+), A(2)(+), and O+ are used for this purpose. Confocal microscopy images show that after 30 min of conjugation time, type A(+) and A(2)(+) erythrocytes present bright emission, whereas the O+ group cells show no emission. Fluorescence intensity maps show different antigen expressions for the distinct erythrocyte types. The results obtained strongly suggest that this simple labeling procedure may be employed as an efficient tool to investigate quantitatively the distribution and expression of antigens in red blood cell membranes. (C) 2005 Society of Photo-Optical Instrumentation Engineers.
Subject: biomedical optics
optical materials
quantum dots
Country: EUA
Editor: Spie-int Society Optical Engineering
Rights: aberto
Identifier DOI: 10.1117/1.1993257
Date Issue: 2005
Appears in Collections:Unicamp - Artigos e Outros Documentos

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