Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/60576
Type: Artigo de periódico
Title: Inulinase Production by Agro-Industrial Residues: Optimization of Pretreatment of Substrates and Production Medium
Author: Sguarezi, C
Longo, C
Ceni, G
Boni, G
Silva, MF
Di Luccio, M
Mazutti, MA
Maugeri, F
Rodrigues, MI
Treichel, H
Abstract: In this work, production of inulinase was studied. Media formulation was optimized by experimental design and response surface techniques, as well as the pretreatment of the agro-industry residues used in the formulation of fermentation medium. Two agro-industry residues were investigated: sugarcane molasses (SCM) and corn steep liquor (CSL). Pretreatment with sulfuric acid was the most effective for clarification of SCM (pH 5.0, 24 h of resting time and final pH 4.0). Clarification of CSL was accomplished with phosphoric acid (pH 3.0, 24 h of resting time and final pH 5.5). A color reduction of approximately 70% was achieved for both substrates. The highest production of inulinase was obtained in a medium containing 100 g l(-1) of pretreated SCM, 100 g l(-1) of pretreated CSL and 6 g l(-1) of Prodex Lac (yeast hydrolysate), yielding 1,139 U ml(-1).
Subject: Agro-industrial residues
Experimental design
Inulinase
Kluyveromyces marxianus
Pretreatment
Sugarcane molasses
Corn steep liquor
Country: EUA
Editor: Springer
Rights: fechado
Identifier DOI: 10.1007/s11947-007-0042-x
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

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