Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/60308
Type: Artigo de periódico
Title: Insulin-Loaded Poly(epsilon-Caprolactone) Nanoparticles: Efficient, Sustained and Safe Insulin Delivery System
Author: de Araujo, TM
Teixeira, Z
Barbosa-Sampaio, HC
Rezende, LF
Boschero, AC
Duran, N
Hoehr, NF
Abstract: The aim of this work was to develop an efficient, biodegradable, biocompatible and safe controlled release system using insulin-loaded poly(epsilon-caprolactone) (PCL) nanoparticles. The insulin-loaded PCL nanoparticles were prepared by double emulsion method (water-in-oil-in-water) using Pluronic F68 as emulsifier. Using the double emulsion method a high insulin encapsulation efficiency (90.6 +/- 1.6%) with a zeta potential of -29 +/- 2.7 mV and average particle size of 796 +/- 10.5 nm was obtained. Insulin-loaded PCL nanoparticles showed no toxicity to MIN6 cells. Insulin nanoparticles administered subcutaneously and intraperitoneally in rats reduced glycaemia of basal levels after 15 minutes, and presented a sustainable hypoglycemic effect on insulin-dependent type 1 diabetic rats, showing to be more efficient than unencapsulated insulin. Furthermore, these nanoparticles were not hepatotoxic, as evaluated by the effect over liver cell-death and oxidative stress scavenger system in rats. These results suggest that insulin-loaded PCL nanoparticles prepared by water-in-oil-in-water emulsion method are biocompatible, efficient and safe insulin-delivering system with controlled insulin release, which indicates that it may be a powerful tool for insulin-dependent patients care.
Subject: Insulin
Diabetes
Polymeric Nanoparticles
Sustained Release
Cytotoxicily
Hepatotoxicity
Country: EUA
Editor: Amer Scientific Publishers
Rights: fechado
Identifier DOI: 10.1166/jbn.2013.1607
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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