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Type: Artigo de periódico
Title: Insulin-induced tyrosine phosphorylation of Shc in liver, muscle and adipose tissue of insulin resistant rats
Author: Paez-Espinosa, EV
Rocha, EM
Velloso, LA
Boschero, AC
Saad, MJA
Abstract: Insulin stimulates rapid tyrosine phosphorylation of the protein Shc, which subsequently binds to Grb2, resulting in the activation of a complex mitogenic signaling network. In this study, we examined the levels of Shc protein, its phosphorylation state and Shc-Grb2 association in liver, muscle and adipose tissue before and after insulin administration in three animal models of insulin resistance (chronic dexamethasone treatment, 72-h starvation and aging). There were no differences in Shc protein expression between tissues from control and insulin resistant animals. In fasted hypoinsulinemic rats, there was a decrease in insulin-induced Shc phosphorylation in liver and adipose tissue.:However, a significant increase in Shc phosphorylation was observed in liver and muscle from dexamethasone-treated hyperinsulinemic rats and in liver, muscle and adipose tissue of hyperinsulinemic 20-month-old rats. Alterations in Shc phosphorylation correlated well with the level of Shc-Grb2 association. These results indicate that Shc tyrosyl phosphorylation and Shc-Grb2 association are regulated in the different types of insulin resistance and that this regulation is apparently related to the animals' plasma insulin levels. The Shc-Grb2 association is directly related to the insulin-induced tyrosyl phosphorylation of Shc. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.
Subject: diabetes
Shc-Grb 2 association
Country: Irlanda
Editor: Elsevier Sci Ireland Ltd
Citation: Molecular And Cellular Endocrinology. Elsevier Sci Ireland Ltd, v. 156, n. 41671, n. 121, n. 129, 1999.
Rights: fechado
Identifier DOI: 10.1016/S0303-7207(99)00137-9
Date Issue: 1999
Appears in Collections:Unicamp - Artigos e Outros Documentos

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