Please use this identifier to cite or link to this item:
http://repositorio.unicamp.br/jspui/handle/REPOSIP/60307
Type: | Artigo de periódico |
Title: | Insulin-induced tyrosine phosphorylation of Shc in liver, muscle and adipose tissue of insulin resistant rats |
Author: | Paez-Espinosa, EV Rocha, EM Velloso, LA Boschero, AC Saad, MJA |
Abstract: | Insulin stimulates rapid tyrosine phosphorylation of the protein Shc, which subsequently binds to Grb2, resulting in the activation of a complex mitogenic signaling network. In this study, we examined the levels of Shc protein, its phosphorylation state and Shc-Grb2 association in liver, muscle and adipose tissue before and after insulin administration in three animal models of insulin resistance (chronic dexamethasone treatment, 72-h starvation and aging). There were no differences in Shc protein expression between tissues from control and insulin resistant animals. In fasted hypoinsulinemic rats, there was a decrease in insulin-induced Shc phosphorylation in liver and adipose tissue.:However, a significant increase in Shc phosphorylation was observed in liver and muscle from dexamethasone-treated hyperinsulinemic rats and in liver, muscle and adipose tissue of hyperinsulinemic 20-month-old rats. Alterations in Shc phosphorylation correlated well with the level of Shc-Grb2 association. These results indicate that Shc tyrosyl phosphorylation and Shc-Grb2 association are regulated in the different types of insulin resistance and that this regulation is apparently related to the animals' plasma insulin levels. The Shc-Grb2 association is directly related to the insulin-induced tyrosyl phosphorylation of Shc. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved. |
Subject: | diabetes fasting p52Shc Shc-Grb 2 association hypoinsulinemia hyperinsulinemia |
Country: | Irlanda |
Editor: | Elsevier Sci Ireland Ltd |
Citation: | Molecular And Cellular Endocrinology. Elsevier Sci Ireland Ltd, v. 156, n. 41671, n. 121, n. 129, 1999. |
Rights: | fechado |
Identifier DOI: | 10.1016/S0303-7207(99)00137-9 |
Date Issue: | 1999 |
Appears in Collections: | Unicamp - Artigos e Outros Documentos |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
WOS000083410600014.pdf | 372 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.