Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/60244
Type: Artigo de periódico
Title: Influence of culture conditions on glutathione production by Saccharomyces cerevisiae
Author: Santos, LO
Gonzales, TA
Ubeda, BT
Alegre, RM
Abstract: A strategy of experimental design using a fractional factorial design (FFD) and a central composite rotatable design (CCRD) were carried out with the aim to obtain the best conditions of temperature (20-30 degrees C), agitation rate (100-300 rpm), initial pH (5.0-7.0), inoculum concentration (5-15%), and glucose concentration (30-70 g/l) for glutathione (GSH) production in shake-flask culture by Saccharomyces cerevisiae ATCC 7754. By a FFD (2(5-2)), the agitation rate, temperature, and pH were found to be significant factors for GSH production. In CCRD (2(2)) was obtained a second-order model equation, and the percent of variation explained by the model was 95%. The results showed that the optimal culture conditions were agitation rate, 300 rpm; temperature, 20 C; initial pH, 5; glucose, 54 g/l; and inoculum concentration, 5%. The highest GSH concentration (154.5 mg/l) was obtained after 72 h of fermentation.
Subject: glutathione
saccharomyces cerevisiae
culture optimization
experimental design
Country: EUA
Editor: Springer
Rights: fechado
Identifier DOI: 10.1007/s00253-007-1211-6
Date Issue: 2007
Appears in Collections:Unicamp - Artigos e Outros Documentos

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