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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titleInfluence of Adhesive Restorations on Diffusion of H2O2 Released from a Bleaching Agent and Its Toxic Effects on Pulp Cellspt_BR
dc.contributor.authorSoares, DGpt_BR
dc.contributor.authorPastana, JVpt_BR
dc.contributor.authorDuque, CCDpt_BR
dc.contributor.authorRibeiro, APDpt_BR
dc.contributor.authorBasso, FGpt_BR
dc.contributor.authorHebling, Jpt_BR
dc.contributor.authorCosta, CADpt_BR
unicamp.authorSoares, Diana Gabriela UNESP, Araraquara Sch Dent, Dept Dent Mat & Prosthodont, Araraquara, SP, Brazilpt_BR
unicamp.authorPastana, Julia Vieira de Oliveira Duque, Carla Caroline de Souza Costa, Carlos Alberto UNESP, Araraquara Sch Dent, Dept Physiol & Pathol, Araraquara, SP, Brazilpt_BR
unicamp.authorDias Ribeiro, Ana Paula Fed Univ Brasilia, Dept Dent, Brasilia, DF, Brazilpt_BR
unicamp.authorBasso, Fernanda Goncalves Univ Estadual Campinas, UNICAMP, Piracicaba Dent Sch, Dept Oral Diag, Piracicaba, SP, Brazilpt_BR
unicamp.authorHebling, Josimeri UNESP, Araraquara Sch Dent, Dept Orthodont & Pediat Dent, Araraquara, SP, Brazilpt_BR
dc.subjecttooth bleachingpt_BR
dc.subject.wosMicrotensile Bond Strengthpt_BR
dc.subject.wosWater Storagept_BR
dc.subject.wosComposite Restorationspt_BR
dc.description.abstractPurpose: To assess the influence of adhesive restorations on hydrogen peroxide (H2O2) diffusion through enamel and dentin and its cytotoxicity to pulp (MDPC-23) cells. Materials and Methods: Sound and resin-restored enamel/dentin disks were stored in water for 24h or 6 months and adapted to artificial pulp chambers. Bleaching gels with 20% or 35% H2O2 were applied to the enamel surface for 45 min, and a culture medium in direct contact with the dentin surface (extract) was applied for 1 h to the MDPC-23 cells. Cell metabolism (MU assay) and cell morphology (SEM) were assessed. The amount of H2O2 in the extracts was also quantified (peroxidase/leuco-crystal violet reaction). Results: A significant reduction in cell metabolism was observed between the group bleached with the 35% gel and the control group (sound, nonbleached) (p < 0.05). The H2O2 diffusion was directly related to its concentration in the bleaching gel. The variables "presence of restoration" and "time of water storage" did not significantly influence H2O2 diffusion or cell metabolism for either of the bleaching gels (p > 0.05). All bleached groups presented alterations in cell morphology related to the concentration of H2O2 in the bleaching gel. Conclusion: The reduction in cell metabolism and the changes in cell morphology were H2O2-concentration dependent, having no relationship with the presence of either new or aged adhesive restorations on teeth subjected to bleaching
dc.relation.ispartofJournal Of Adhesive Dentistrypt_BR
dc.relation.ispartofabbreviationJ. Adhes. Dent.pt_BR
dc.publisher.cityHanover Parkpt_BR
dc.publisherQuintessence Publishing Co Incpt_BR
dc.identifier.citationJournal Of Adhesive Dentistry. Quintessence Publishing Co Inc, v. 16, n. 2, n. 123, n. 128, 2014.pt_BR
dc.sourceWeb of Sciencept_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorship1Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorship1Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsordocumentnumberFAPESP [2011/12938-8, 2011/09385-7]pt
dc.description.sponsordocumentnumberCNPq [301029/2010-1]pt
dc.description.provenanceMade available in DSpace on 2014-07-30T14:33:31Z (GMT). No. of bitstreams: 0 Previous issue date: 2014en
dc.description.provenanceMade available in DSpace on 2015-11-26T16:33:44Z (GMT). No. of bitstreams: 0 Previous issue date: 2014en
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