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Type: Artigo de periódico
Title: Inflammatory oedema induced by Lachesis muta muta (Surucucu) venom and LmTX-I in the rat paw and dorsal skin
Author: Ferreira, T
Camargo, EA
Ribela, MTCP
Damico, DC
Marangoni, S
Antunes, E
De Nucci, G
Landucci, ECT
Abstract: The ability of crude venom and a basic phospholipase A(2) (LmTX-I) from Lachesis muta muta venom to increase the microvascular permeability in rat paw and skin was investigated. Crude venom or LmTX-I were injected subplantarly or intradermally and rat paw oedema and dorsal skin plasma extravasation were measured. Histamine release from rat peritoneal mast cell was also assessed. Crude venom or LmTX-I induced dose-dependent rat paw oedema and dorsal skin plasma extravasation. Venom-induced plasma extravasation was inhibited by the histamine H(1) antagonist mepyramine (6 mg/kg), histamine/5-hydroxy-triptamine antagonist cyproheptadine (2 rng/kg), cyclooxygenase inhibitor indomethacin (5 mg/kg), nitric oxide synthesis inhibitor L-NAME (100 nmol/site), tachykinin NK(1) antagonist SR140333 (1 nmol/site) and bradykinin B(2) receptor antagonist Icatibant (0.6 mg/kg). Platelet-activating factor (PAF) antagonist PCA4248 (5 mg/kg) had no effect. LmTX-I-induced skin extravasation was inhibited by cyproheptadine, mepyramine, indomethacin and PCA4248, while L-NAME and SR140333 had no effect. Additionally, both Lachesis muta muta venom and LmTX-I concentration-dependently induced histamine release from rat mast cells. In conclusion, Lachesis muta muta venom and LmTX-I increase microvascular permeability by mechanisms involving in vivo mast cell activation and arachidonic acid metabolites. Additionally, crude venom-induced responses also involve substance P, nitric oxide and bradykinin release, whether LmTX-I-induced responses involve PAF. (c) 2008 Published by Elsevier Ltd.
Subject: Snake venom
Vascular permeability
Mast cells
Sensory fibres
Lachesis muta muta
Country: Inglaterra
Editor: Pergamon-elsevier Science Ltd
Rights: fechado
Identifier DOI: 10.1016/j.toxicon.2008.10.016
Date Issue: 2009
Appears in Collections:Unicamp - Artigos e Outros Documentos

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