Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/60105
Type: Artigo de periódico
Title: Induction of protein catabolism and the ubiquitin-proteasome pathway by mild oxidative stress
Author: Gomes-Marcondes, MCC
Tisdale, MJ
Abstract: Muscle wasting in cancer cachexia is associated with increased levels of malondialdehyde (MDA) in gastrocnemius muscles, suggesting an increased oxidative stress. To determine whether oxidative stress contributes to muscle protein catabolism, an in vitro model system, consisting Of C2C12 myotubes, was treated with either 0.2 mM FeSO4, 0.1 mM H2O2, or both, to replicate the rise in MDA content in cachexia. All treatments caused an increased protein catabolism and a decreased myosin expression. There was an increase in the proteasome chymotrypsin-like enzyme activity, while immunoblotting showed an increased expression of the 20S proteasome alpha-subunits. p42, and the ubiquitin-conjugating enzyme, E2(14k). These results show that mild oxidative stress increases protein degradation in skeletal muscle by causing an increased expression of the major components of the ubiquitin-proteasome pathway. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
Subject: oxidative stress
proteolysis
proteasome expression
Country: Irlanda
Editor: Elsevier Sci Ireland Ltd
Rights: fechado
Identifier DOI: 10.1016/S0304-3835(02)00006-X
Date Issue: 2002
Appears in Collections:Unicamp - Artigos e Outros Documentos

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