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Type: Artigo de periódico
Title: Inducible-NOS but not neuronal-NOS participate in the acute effect of TNF-alpha on hypothalamic insulin-dependent inhibition of food intake
Author: Moraes, JC
Amaral, ME
Picardi, PK
Calegari, VC
Romanatto, T
Bermudez-Echeverry, M
Chiavegatto, S
Saad, MJ
Velloso, LA
Abstract: TNF-alpha acts on the hypothalamus modulating food intake and energy expenditure through mechanisms incompletely elucidated. Here, we explore the hypothesis that, to modulate insulin-induced anorexigenic signaling in hypothalamus, TNF-a requires the synthesis of NO. TNF-alpha activates signal transduction through JNK and p38 in hypothalamus, peaking at 10(-8) M. This is accompanied by the induction of expression of the inducible and neuronal forms of NOS, in both cases peaking at 10(-12) M. In addition, TNF-alpha stimulates NOS catalytic activity. Pre-treatment with TNF-alpha at a low dose (10(-12) M) inhibits insulin-dependent anorexigenic signaling, and this effect is abolished in iNOS but not in nNOS knockout mice. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Subject: cytokine
food intake
nitric oxide
nitric oxide synthase
tumor necrosis factor
Country: Holanda
Editor: Elsevier Science Bv
Citation: Febs Letters. Elsevier Science Bv, v. 580, n. 19, n. 4625, n. 4631, 2006.
Rights: fechado
Identifier DOI: 10.1016/j.febslet.2006.07.042
Date Issue: 2006
Appears in Collections:Unicamp - Artigos e Outros Documentos

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