Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/59633
Type: Artigo de periódico
Title: Photoinactivation of different human tumor cell lines and sheep red blood cells in vitro by liposome-bound Zn(II) Phthalocyanine: Effects of cholesterol
Author: de Oliveira, CA
Kohn, LK
Antonio, MA
Carvalho, JE
Moreira, MR
Machado, AEH
Pessine, FBT
Abstract: The in vitro photoinactivation of human tumor cell lines and sheep red blood cells (SRBC) by Zinc (II) Phthalocyanine (ZnPc) was investigated using unilamellar liposome (LUV) as delivery system, in the presence and absence of cholesterol (CHOL) in the formulation. The presence of CHOL improves the stability of the system showing to be essential for the photodynamic action of ZnPc. LUVs prepared without CHOL did not present any antiproliferative effects neither induced significant photohaemolysis. The presence of ZnPc in the culture medium caused total cell growth inhibition (TGI) only at concentrations higher than 250 mu mol dm(-3). For ZnPc in LUV/CHOL (mass ratio = 3:1), the mean TGI values for almost all studied cells were around 80 mu mol dm(-3), and 14 mu mol dm(-3) for human ovarian carcinoma (NIH: OVCAR-3) cells. The cytoplasmic components of OVCAR-3 and SRBC when irradiated in presence of ZnPc in LUV/CHOL were completely destroyed, culminating in cell swelling, lysis and death by necrosis. (C) 2010 Elsevier B.V. All rights reserved.
Subject: Liposome-bound Zn(II) Phthalocyanine
Photodynamic therapy
Tumor cell lines
Sheep red blood cells
Cholesterol
Photohaemolysis and antiproliferative effects
Country: Suíça
Editor: Elsevier Science Sa
Rights: fechado
Identifier DOI: 10.1016/j.jphotobiol.2010.05.006
Date Issue: 2010
Appears in Collections:Unicamp - Artigos e Outros Documentos

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