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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titlePhotodynamic activity of protoporphyrin IX in Harderian glands of Wistar rats: Monitoring by gland fluorescencept_BR
dc.contributor.authorReis, ERpt_BR
dc.contributor.authorMetze, Kpt_BR
dc.contributor.authorNicola, EMDpt_BR
dc.contributor.authorNicola, JHpt_BR
dc.contributor.authorBorissevitch, IEpt_BR
unicamp.author.emailedmyreis@fcm.unicamp.brpt_BR
unicamp.authorReis, E. R. Nicola, E. M. D. Nicola, J. H. Univ Estadual Campinas, Fac Ciencias Med, Lab Laser, Nucleo Med & Cirurgia Expt, Campinas, SP, Brazilpt_BR
unicamp.authorMetze, K. Univ Estadual Campinas, Fac Ciencias Med, Lab Anat Patol, Nucleo Med & Cirurgia Expt, Campinas, SP, Brazilpt_BR
unicamp.authorBorissevitch, I. E. Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Fis, Lab Fotobiofis, Ribeirao Preto, SP, Brazilpt_BR
dc.subjectPhotodynamic therapypt_BR
dc.subjectIn vivo experimental modelspt_BR
dc.subjectWistar rat Harderian glandspt_BR
dc.subjectFluorescence spectrum evolutionpt_BR
dc.subjectTissue degradation factorpt_BR
dc.subject.wosSinglet Oxygenpt_BR
dc.subject.wosTherapypt_BR
dc.description.abstractThis paper presents results on Wistar rat Harderian glands photodegradation experimental study under visible light. Tests were conducted on 20 rats and a 632.8 nm He-Ne laser light was utilized for gland irradiation. The tissue degradation was evaluated by the microphotograph analysis, which demonstrated progressive disappearance of the acinus architecture and a increase in number of nuclei in the pyknotic form, characteristic for the cell degradation. At the same time, the intensities of initial fluorescence peaks at 636.2 nm and 705.8 nm, were diminished with increase in irradiation time and formation of a new peak with a maximum at 680 nm was observed. The systematic observation of the Harderian gland fluorescence spectrum evolutions in comparison with its tissue degradation factor demonstrated high occurrence of the result repetition for different animals even at very low light fluencies. This fact makes it possible to evaluate the tissue photodegradation level via monitoring of its fluorescence spectra as a function of the irradiation time and suggests that the Harderian glands provide an excellent experimental model for the study of the photodynamic process in vivo. Analysis of the Harderian gland fluorescence spectra evolution under light irradiation in comparison with that of protoporphyrin IX in solutions demonstrated that it is protoporphyrin IX responsible for gland fluorescence and photodynamic degradation. The formation of singlet oxygen under light irradiation of protoporphyrin IX solutions demonstrated that the principal mechanism of gland photodegradation is the Type II photodynamic. (C) 2012 Elsevier B.V. All rights reserved.pt
dc.relation.ispartofJournal Of Luminescencept_BR
dc.relation.ispartofabbreviationJ. Lumines.pt_BR
dc.publisher.cityAmsterdampt_BR
dc.publisher.countryHolandapt_BR
dc.publisherElsevier Science Bvpt_BR
dc.date.issued2013pt_BR
dc.date.monthofcirculationMAYpt_BR
dc.identifier.citationJournal Of Luminescence. Elsevier Science Bv, v. 137, n. 32, n. 36, 2013.pt_BR
dc.language.isoenpt_BR
dc.description.volume137pt_BR
dc.description.firstpage32pt_BR
dc.description.lastpage36pt_BR
dc.rightsfechadopt_BR
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policypt_BR
dc.sourceWeb of Sciencept_BR
unicamp.cruespUSPpt_BR
dc.identifier.issn0022-2313pt_BR
dc.identifier.wosidWOS:000316832600006pt_BR
dc.identifier.doi10.1016/j.jlumin.2012.11.032pt_BR
dc.description.sponsorshipFAEP/UNICAMPpt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorship1Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorship1Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.date.available2014-07-30T14:31:44Z
dc.date.available2015-11-26T17:37:39Z-
dc.date.accessioned2014-07-30T14:31:44Z
dc.date.accessioned2015-11-26T17:37:39Z-
dc.description.provenanceMade available in DSpace on 2014-07-30T14:31:44Z (GMT). No. of bitstreams: 0 Previous issue date: 2013en
dc.description.provenanceMade available in DSpace on 2015-11-26T17:37:39Z (GMT). No. of bitstreams: 0 Previous issue date: 2013en
dc.identifier.urihttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/59600
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/59600-
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