Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/59531
Type: Artigo de periódico
Title: Phosphoproteome Reveals an Atlas of Protein Signaling Networks During Osteoblast Adhesion
Author: Milani, R
Ferreira, CV
Granjeiro, JM
Paredes-Gamero, EJ
Silva, RA
Justo, GZ
Nader, HB
Galembeck, E
Peppelenbosch, MP
Aoyama, H
Zambuzzi, WF
Abstract: Cell adhesion on surfaces is a fundamental process in the emerging biomaterials field and developmental events as well. However, the mechanisms regulating this biological process in osteoblasts are not fully understood. Reversible phosphorylation catalyzed by kinases is probably the most important regulatory mechanism in eukaryotes. Therefore, the goal of this study is to assess osteoblast adhesion through a molecular prism under a peptide array technology, revealing essential signaling proteins governing adhesion-related events. First, we showed that there are main morphological changes on osteoblast shape during adhesion up to 3 h. Second, besides classical proteins activated upon integrin activation, our results showed a novel network involving signaling proteins such as Rap1A, PKA, PKC, and GSK3 beta during osteoblast adhesion on polystyrene. Third, these proteins were grouped in different signaling cascades including focal adhesion establishment, cytoskeleton rearrangement, and cell-cycle arrest. We have thus provided evidence that a global phosphorylation screening is able to yield a systems-oriented look at osteoblast adhesion, providing new insights for understanding of bone formation and improvement of cell-substratum interactions. Altogether, these statements are necessary means for further intervention and development of new approaches for the progress of tissue engineering. Cell. Biochem. 109: 957-966, 2010. (C) 2010 Wiley-Liss, Inc.
Subject: OSTEOBLAST
CELL ADHESION
SIGNAL TRANSDUCTION
SURFACES
KINOME
PKC
RAP1
BIOENGINEERING
Country: EUA
Editor: Wiley-liss
Rights: fechado
Identifier DOI: 10.1002/jcb.22479
Date Issue: 2010
Appears in Collections:Unicamp - Artigos e Outros Documentos

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