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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titlePharmacological evidence for a presynaptic action of venoms from Bothrops insularis (jararaca ilhoa) and Bothrops neuwiedi (jararaca pintada)pt_BR
dc.contributor.authorRodrigues-Simioni, Lpt_BR
dc.contributor.authorZamuner, SRpt_BR
dc.contributor.authorCogo, JCpt_BR
dc.contributor.authorBorja-Oliveira, CRpt_BR
dc.contributor.authorPrado-Franceschi, Jpt_BR
dc.contributor.authorda Cruz-Hofling, MApt_BR
dc.contributor.authorCorrado, APpt_BR
unicamp.authorUniv Estadual Campinas, UNICAMP, Fac Med Sci, Dept Pharmacol, BR-13081970 Campinas, SP, Brazil Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Histol & Embryol, BR-13083970 Campinas, SP, Brazil UNIVAP, Inst Res & Dev IP & D, BR-12244000 Sao Jose Dos Campos, SP, Brazil Univ Sao Paulo, Fac Med, Dept Pharmacol, BR-13049900 Ribeirao Preto, SP, Brazilpt_BR
dc.subjectbiventer cervicis preparationpt_BR
dc.subjectBothrops venomspt_BR
dc.subjectneuromuscular blockadept_BR
dc.subject.wosNerve-muscle Preparationpt_BR
dc.subject.wosPhospholipase A(2)pt_BR
dc.description.abstractWhereas the presynaptic action of Crotalus durissus terrificus venom is well-established, Bothrops venoms have historically been considered to have only postsynaptic and muscular effects. However, some studies have also suggested a presynaptic action for these venoms. In this work, we used chick biventer cervicis preparations to compare the presynaptic actions of two Bothrops venoms (B. insularis and B. neuwiedi) with that of C. d. terrificus venom. At 10 mug/ml, all venoms produced irreversible blockade of the twitch tension responses, with no reduction in acetylcholine (ACh)-induced contractures and only a slight decrease in potassium induced-contractures. The times (in min) required to produce 50% neuromuscular blockade (C. d. terrificus: 16.3 +/- 0.7, n = 8; B. insularis: 30.0 +/- 1.9, n = 5; B. neuwiedi: 42.0 +/- 2.0, n = 8 mean +/- SEM) were significantly different among the venoms (p < 0.01). Lowering the temperature at which the experiments were done (from 37 to 24 degreesC) prevented neuromuscular blockade by the three venoms, indicating that enzyme activity may be involved in this response. At concentrations capable of causing complete neuromuscular blockade, creatine kinase release remained close to levels seen ill control preparations incubated with Krebs solution alone (500-1200 IU/l). Commercial crotalic antivenom, but not bothropic antivenom, protected against the neuromuscular blockade caused by B. insularis and B. neuwiedi venoms. These observations indicate that bothropic venoms may contain components which act presynaptically in a manner similar to C. d. terrificus venom, and that at low venom concentrations a direct action oil skeletal muscle does not contribute to this presynaptic neurotoxicity. (C) 2003 Elsevier Ltd. All rights
dc.publisherPergamon-elsevier Science Ltdpt_BR
dc.identifier.citationToxicon. Pergamon-elsevier Science Ltd, v. 43, n. 6, n. 633, n. 638, 2004.pt_BR
dc.sourceWeb of Sciencept_BR
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