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Type: | Artigo |
Title: | Pharmacological characterization of the rat paw edema induced by Bothrops lanceolatus (Fer de lance) venom |
Title Alternative: | |
Author: | Bon, C. Araujo, A.L. de Antunes, E. Faria, L. de |
Abstract: | The inflammatory response induced by Bothrops lanceolatus venom (BLV) in the rat hind-paw was studied measuring paw edema. Non-heated BLV (75 mug/paw) caused a marked paw edema accompanied by intense haemorrhage whereas heated venom (97 degreesC, 30 s; 12.5-100 mug/paw) produced a dose- and time-dependent non-haemorrhage edema. The response with heated BLV was maximal within 15 min disappearing over 24 h. Heated venom was then routinely used at the dose of 75 mug/paw. The prostacyclin analogue iloprost (0.1 mug/paw) potentiated by 125% the venom-induced edema. The histamine H-1 receptor antagonist mepyramine (6 mg/kg) or the serotonin/histamine receptor antagonist cyproheptadine (6 mg/kg) partially inhibited BLV-induced edema whereas the combination of both compounds virtually abolished the edema. The lipoxygenase inhibitor BWA4C (10 mg/kg), but not the cyclooxygenase inhibitor indomethacin (10 mg/kg), significantly inhibited the edema (35% reduction; P < 0.05). Dexamethasone (1 mg/kg) also markedly (P < 0.001) reduced venom-induced edema. The brady-kinin B-2 receptor antagonist Hoe 140 (0.6 mg/kg) reduced by 30% (P < 0.05) the venom induced edema, whereas the angiotensin-converting enzyme inhibitor captopril (300 <mu>g/paw) potentiated by 42% (P < 0.05) the edema. Bothrops lanceolatus antivenon (anti-BLV) reduced by 28% (P < 0.05) the venom-induced edema while intravenous administration of antivenom failed to affect the edema. In conclusion, BLV-induced rat paw edema involves mast cell degranulation causing local release of histamine and serotonin, a phenomenon mediated mainly by kinins and lipoxygenase metabolites. Additionally, the use of a specific Bothrops lanceolatus antivenom, given subplantarily or intravenously, revealed to be little effective to prevent BLV-induced edema |
metadata.dc.description.abstractalternative: | |
Subject: | Bothrops lanceolatus Antivenenos |
Country: | Reino Unido |
Editor: | Elsevier |
Citation: | Toxicon. Pergamon-elsevier Science Ltd, v. 39, n. 6, n. 825, n. 830, 2001. |
Rights: | fechado |
Identifier DOI: | 10.1016/S0041-0101(00)00213-0 |
Address: | https://www.sciencedirect.com/science/article/pii/S0041010100002130 |
Date Issue: | 2001 |
Appears in Collections: | FCM - Artigos e Outros Documentos |
Files in This Item:
File | Description | Size | Format | |
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WOS000166445100012.pdf | 156.34 kB | Adobe PDF | View/Open |
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