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Type: Artigo de periódico
Title: Perforin and granzyme B involvement in oral lesions of lichen planus and chronic GVHD
Author: Pimentel, VN
de Matos, LS
Soares, TCB
Adam, R
Metze, K
Correa, MEP
de Souza, CA
Cintra, ML
Abstract: Background: Oral lesions of lichen planus and chronic graft-vs.-host disease (cGVHD) have similar clinical and histological features, but distinct etiology. Apoptosis induced by cytotoxic T lymphocyte has been proposed as a mechanism of keratinocytes death. Cytotoxicity can be mediated by granules containing granzyme B and perforin. Since common features can reflect similarities in immunological mechanisms, we studied the role of those molecules in both diseases. Methods: We analyzed 29 cases of oral lichen planus and 27 of oral cGVHD. The sections were studied on H&E, perforin and granzyme B staining. Results: The total means (epithelium plus connective tissue number) of the granzyme B- and perforin-positive cells were significantly higher in cGVHD than in oral lichen planus lesions (P < 0.05). Also, it was found that the higher the number of perforin+ cells, the higher the number of granzyme-B+ cells in the epithelium and in the connective tissue for both groups (P < 0.05). In oral lichen planus, the number of single apoptotic bodies had a positive correlation with connective tissue granzyme immunostaining and a negative correlation with perforin (P < 0.01). On the contrary, in oral cGVHD, the number of apoptotic body clusters presented a positive correlation with connective tissue perforin (P < 0.01). Conclusions: Our findings indicate that apoptosis in oral lichen planus seems to be correlated with granzyme B release, while in oral cGVHD, perforin seems to be more important. Although these diseases present clinical and histological similarities, subtle differences seem to exist in their pathogenetic mechanisms.
Subject: apoptosis
bone marrow transplantation
cytotoxic granules
Country: EUA
Editor: Wiley-blackwell
Citation: Journal Of Oral Pathology & Medicine. Wiley-blackwell, v. 39, n. 10, n. 741, n. 746, 2010.
Rights: fechado
Identifier DOI: 10.1111/j.1600-0714.2010.00917.x
Date Issue: 2010
Appears in Collections:Unicamp - Artigos e Outros Documentos

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