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Type: Artigo de periódico
Title: PEG plus potassium phosphate plus urea aqueous two-phase systems: Phase equilibrium and protein partitioning
Author: da Silva, LHM
Meirelles, AJA
Abstract: Liquid-liquid equilibria of poly(ethylene glycol) (PEG) + potassium phosphate + urea + water aqueous two-phase-systems were studied for different PEG molecular weights and pHs. Experimental techniques and analytical methods are described. Equilibrium data were obtained for PEG 1450, 3350, 10000) + potassium phosphate (pH 7 and 9) + urea (6 mass%) + water aqueous two-phase systems at 25 degreesC. Four tie lines were measured for each system. The partitioning behaviors of lysozyme, catalase, and beta -galactosidase were studied in these systems at 25 degreesC, at pH 7 and 9, and at the urea. concentrations 3 and 6 mass %.
Country: EUA
Editor: Amer Chemical Soc
Rights: fechado
Identifier DOI: 10.1021/je000131f
Date Issue: 2001
Appears in Collections:Unicamp - Artigos e Outros Documentos

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