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|Type:||Artigo de periódico|
|Title:||PEG plus potassium phosphate plus urea aqueous two-phase systems: Phase equilibrium and protein partitioning|
|Author:||da Silva, LHM|
|Abstract:||Liquid-liquid equilibria of poly(ethylene glycol) (PEG) + potassium phosphate + urea + water aqueous two-phase-systems were studied for different PEG molecular weights and pHs. Experimental techniques and analytical methods are described. Equilibrium data were obtained for PEG 1450, 3350, 10000) + potassium phosphate (pH 7 and 9) + urea (6 mass%) + water aqueous two-phase systems at 25 degreesC. Four tie lines were measured for each system. The partitioning behaviors of lysozyme, catalase, and beta -galactosidase were studied in these systems at 25 degreesC, at pH 7 and 9, and at the urea. concentrations 3 and 6 mass %.|
|Editor:||Amer Chemical Soc|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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