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Type: Artigo de periódico
Title: Partitioning optimization of proteins from Zea mays malt in ATPS PEG 6000/CaCl2
Author: Ferreira, GB
Evangelista, AF
Severo, JB
de Souza, RR
Santana, JCC
Tambourgi, EB
Jordao, E
Abstract: This work aimed to establish the relationship between the compositions and pH of ATPS PEG 6000/CaCl2 and the proteins partition from maize malt and also to simplify the process optimization in ATPS for a statistical model, established by response surface methodology (RSM). Results showed that these were no influence of pH on the phase diagrams and on the composition of tie line length of PEG 6000/CaCl2 ATPS. SRM analyses showed that elevated pH and larger tie line length were the best conditions for recovering of maize malt proteins. The maximum partition coefficient by PEG 6000/CaCl2 ATPS was about 4.2 and was achieved in ATPS in a single purification step. The theoretical maximum partition coefficient was between 4.1-4.3. The process was very suitable for continuous aqueous two phase purification due to the stability of proteins (e.g. alpha and beta-amylases) and could increase their content into middle.
Subject: partitioning
aqueous two-phase systems
maize malt
PEG 6000
Country: Brasil
Editor: Inst Tecnologia Parana
Rights: aberto
Identifier DOI: 10.1590/S1516-89132007000300020
Date Issue: 2007
Appears in Collections:Unicamp - Artigos e Outros Documentos

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