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|Type:||Artigo de periódico|
|Title:||Partitioning of pectinolytic enzymes in polyethylene glycol/potassium phosphate aqueous two-phase systems|
|Abstract:||Four different pectinolytic enzymes have been partitioned in polyethylene glycol (PEG)-potassium phosphate aqueous two-phase systems (20-15%). The influence of PEG molecular weight and the addition of sodium chloride were investigated. The best results concerning to the partition coefficients (K-p and K-e) and the yield recovery (R) were: PEG-400/phosphate with NaCl for exo-polygalacturonase (K-e = 5.35 and R = 89.5%), PEG-600/phosphate for pectin lyase (PL) (K-e = 43.18 and R = 98.5%) and pectinesterase (PE) (K-e = 1.51 and R 69.6%), and PEG-10,000/phosphate for endo-polygalacturonase (K-e = 1.35 and R = 53.5%). The best purification factors were observed in the upper phase for the systems containing high molecular weight PEG without NaCl: PEG-6000 for exo-polygalacturonase (5.49 fold) and PEG-10,000 for endo-polygalacturonase (16.28 fold), PE (16.64 fold) and PL (14.27 fold). (C) 2002 Elsevier Science Ltd. All rights reserved.|
aqueous two-phase systems
|Editor:||Elsevier Sci Ltd|
|Appears in Collections:||Unicamp - Artigos e Outros Documentos|
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