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Type: Artigo de periódico
Title: Partitioning of pectinolytic enzymes in polyethylene glycol/potassium phosphate aqueous two-phase systems
Author: Lima, AS
Alegre, RM
Meirelles, AJA
Abstract: Four different pectinolytic enzymes have been partitioned in polyethylene glycol (PEG)-potassium phosphate aqueous two-phase systems (20-15%). The influence of PEG molecular weight and the addition of sodium chloride were investigated. The best results concerning to the partition coefficients (K-p and K-e) and the yield recovery (R) were: PEG-400/phosphate with NaCl for exo-polygalacturonase (K-e = 5.35 and R = 89.5%), PEG-600/phosphate for pectin lyase (PL) (K-e = 43.18 and R = 98.5%) and pectinesterase (PE) (K-e = 1.51 and R 69.6%), and PEG-10,000/phosphate for endo-polygalacturonase (K-e = 1.35 and R = 53.5%). The best purification factors were observed in the upper phase for the systems containing high molecular weight PEG without NaCl: PEG-6000 for exo-polygalacturonase (5.49 fold) and PEG-10,000 for endo-polygalacturonase (16.28 fold), PE (16.64 fold) and PL (14.27 fold). (C) 2002 Elsevier Science Ltd. All rights reserved.
Subject: pectinase
commercial enzyme
aqueous two-phase systems
Country: Inglaterra
Editor: Elsevier Sci Ltd
Rights: fechado
Identifier DOI: 10.1016/S0144-8617(01)00376-9
Date Issue: 2002
Appears in Collections:Unicamp - Artigos e Outros Documentos

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