Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/59046
Type: Artigo de periódico
Title: Parameters optimization for enzymatic assays using experimental design
Author: Burkert, JFM
Kalil, SJ
Maugeri, F
Rodrigues, MI
Abstract: The conditions for maximization enzymatic activity were determined using experimental design and inulinase from Kluyveromyces marxianus ATCC 16045. The effects of substrate concentration (sucrose and inulin), pH and temperature on inulinase activity were verified using four factorial design and surface response analysis. Using sucrose as substrate. It has bean shown that the effects sucrose on enzymatic activity is not statistically significant and the best condition for the highest activity (110 U/mL) was achieved with temperature between 60 degrees C and 68 degrees C and pH between 4.5 and 5.0. Using inulin as substrate it was verified that temperature is the only variable statistically significant and the maximum activity was 7.3 U/mL at temperature between 50 degrees C and 51 degrees C.
Subject: inulinase
Kluyveromyces marxianus
activity
factorial design and response surface analysis
Country: Brasil
Editor: Brazilian Soc Chemical Eng
Rights: aberto
Date Issue: 2006
Appears in Collections:Unicamp - Artigos e Outros Documentos

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