Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/58840
Type: Artigo de periódico
Title: Raman spectroscopy and chemometrics for on-line control of glucose fermentation by Saccharomyces cerevisiae
Author: Avila, TC
Poppi, RJ
Lunardi, I
Tizei, PAG
Pereira, GAG
Abstract: This work presents the use of Raman spectroscopy and chemometrics for on-line control of the fermentation process of glucose by Saccharomyces cerevisiae. In a first approach, an on-line determination of glucose, ethanol, glycerol, and cells was accomplished using multivariate calibration based on partial least squares (PLS). The PLS models presented values of root mean square error of prediction (RMSEP) of 0.53, 0.25, and 0.02% for glucose, ethanol and glycerol, respectively, and RMSEP of 1.02 g L-1 for cells. In a second approach, multivariate control charts based on multiway principal component analysis (MPCA) were developed for detection of fermentation fault-batch. Two multivariate control charts were developed, based on the squared prediction error (Q) and Hotelling's T2. The use of the Q control chart in on-line monitoring was efficient for detection of the faults caused by temperature, type of substrate and contamination, but the T2 control chart was not able to monitor these faults. On-line monitoring by Raman spectroscopy in conjunction with chemometric procedures allows control of the fermentative process with advantages in relation to reference methods, which require pretreatment, manipulation of samples and are time consuming. Also, the use of multivariate control charts made possible the detection of faults in a simple way, based only on the spectra of the system. (C) 2012 American Institute of Chemical Engineers Biotechnol. Prog., 2012
Subject: on-line monitoring
Raman spectroscopy
partial least squares
multiway principal component analysis
multivariate control charts
Country: EUA
Editor: Wiley-blackwell
Rights: aberto
Identifier DOI: 10.1002/btpr.1615
Date Issue: 2012
Appears in Collections:Unicamp - Artigos e Outros Documentos

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