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Type: Artigo de periódico
Title: Quantification of Lactobacillus in fermented milk by multivariate image analysis with least-squares support-vector machines
Author: Borin, A
Ferrao, MF
Mello, C
Cordi, L
Pataca, LCM
Duran, N
Poppi, RJ
Abstract: This paper reports an approach for quantification of Lactobacillus in fermented milk, grown in a selective medium (MRS agar), by use of digital colour images of Petri plates easily obtained by use of a flatbed scanner. A one-dimensional data vector was formed to characterize each digital image on the basis of the frequency-distribution curves of the red (R), green (G), and blue (B) colour values, and quantities derived from them, for example lightness (L), relative red (RR), relative green (RG), and relative blue (RB). The frequency distributions of hue, saturation, and intensity (HSI) were also calculated and included in the data vector used to describe each image. Multivariate non-linear modelling using the least-squares support vector machine (LS-SVM) and a linear model based on PLS regression were developed to relate the microbiological count and the frequency vector. Feasibly models were developed using the LS-SVM and errors were below than 10% for Lactobacillus quantification, indicating the proposed approach can be used for automatic counting of colonies.
Subject: multivariate image analysis
fermented milk
least-squares support vector machines
Country: Alemanha
Editor: Springer Heidelberg
Citation: Analytical And Bioanalytical Chemistry. Springer Heidelberg, v. 387, n. 3, n. 1105, n. 1112, 2007.
Rights: fechado
Identifier DOI: 10.1007/s00216-006-0971-7
Date Issue: 2007
Appears in Collections:Unicamp - Artigos e Outros Documentos

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