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Type: Artigo de periódico
Title: Purification of microbial beta-galactosidase from Kluyveromyces fragilis by bioaffinity partitioning
Author: Silva, ME
Franco, TT
Abstract: This work investigated the partitioning of beta -galactosidase from Kluyveromyces fragilis in aqueous two-phase systems (ATPS) by bioaffinity. PEG 4000 was chemically activated with thresyl chloride, and the biospecific ligand p-aminophenyl 1-thio-beta -D-galactopyranoside (APGP) was attached to the activated PEG 4000. A new two-step method for extraction and purification of the enzyme beta -galactosidase from Kluyveromyces fragilis was developed. In the first step, a system composed of 6% PEG 4000-APGP and 8% dextran 505 was used, where beta -galactosidase was strongly partitioned to the top phase (K = 2,330). In the second step, a system formed of 13% PEG-APGP and 9% phosphate salt was used to revert the value of the partition coefficient of beta -galactosidase (K = 2 x 10(-5)) in order to provide the purification and recovery of 39% of the enzyme in the bottom salt-rich phase.
Subject: beta-galactosidase
aqueous two-phase systems
protein purification
Country: Brasil
Editor: Soc Brasileira Microbiologia
Rights: aberto
Date Issue: 1999
Appears in Collections:Unicamp - Artigos e Outros Documentos

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