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Type: Artigo de periódico
Title: Purification of Legumin-Like Proteins from Coffea arabica and Coffea racemosa Seeds and Their Insecticidal Properties toward Cowpea Weevil (Callosobruchus maculatus) (Coleoptera: Bruchidae)
Author: Coelho, MB
Macedo, MLR
Marangoni, S
da Silva, DS
Cesarino, I
Mazzafera, P
Abstract: Legumin-like proteins from seeds of Coffea arabica (CaL-1 and CaL-2) and Coffea racemosa (CrL-1 and CrL-2) were characterized and isolated by gel filtration and reverse-phase chromatography. The insecticidal properties of the purified proteins were tested against Callosobruchus maculatus using artificial diets. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analyses indicated that CaL-1 is composed of two subunits of 33 and 24 kDa, while CaL-2, CrL-1, and CrL-2 were monomeric with a single band of 14 kDa. The LD(50) values were 0.5% (w/w) for CaL-1 and 0.3% (w/w) for CaL-2, CrL-1, and CrL-2. ED(50) at 0.3% was assessed for all protein concentrations. The legumin-like proteins were not digested by midgut homogenates of C. maculatus until 8 h of incubation. CaL-1 and CaL-2 (C. arabica) and CrL-1 and CrL-2 (C. racemosa) are chitin-binding proteins, and their insecticidal properties toward C. maculatus larvae might be related to their capacity to bind chitin present in the larval gut and their associated low digestibility.
Subject: Coffee
Country: EUA
Editor: Amer Chemical Soc
Rights: fechado
Identifier DOI: 10.1021/jf9037216
Date Issue: 2010
Appears in Collections:Unicamp - Artigos e Outros Documentos

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