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Type: Artigo de periódico
Title: Purification of amylases from Zea mays malt
Author: Biazus, JPM
Santana, JCC
de Souza, RR
Tambourgi, EB
Abstract: In the present work the purification of a and P-amylase enzymes of maize malt was carried out by expanded bed adsorption and ethanol fractionation downstream processes. 5 mL of 2% maize malt solution in 0.05 M phosphate buffer at pH was injected into a chromatographic column containing Amberlite 410 ion-exchange resin, on a 4 cm fixed bed fluidized to 8 cm. 0.25 M NaCl solution at 14 mL/ minuto of descending flux was used for promotion of the elution, the eluted matter was fractioned by 40-70, 50-80 and 60-90% ethyl alcohol concentration at 20 and 30 degrees C. Activity recovery and purification factor were of 95% and 23 times, respectively, in the first step. 50-80% alcohol concentration at 20 degrees C was the best condition, in which a purification factor of more than 100 times was achieved.
Subject: expanded bed adsorption
corn malt
ethyl alcohol
ion-exchange resin
Country: Brasil
Editor: Soc Brasileira Ciencia Tecnologia Alimentos
Rights: aberto
Identifier DOI: 10.1590/S0101-20612010000100032
Date Issue: 2010
Appears in Collections:Unicamp - Artigos e Outros Documentos

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