Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/58473
Type: Artigo de periódico
Title: Purification of alpha-galactosidase from seeds of Sesbania marginata
Author: Falco, ALP
Durrant, LR
Franco, TT
Abstract: Alpha-galactosidase taken from a raw extract of Sesbania marginata legume seeds was purified by partitioning in aqueous two-phase systems (ATPS). Initially, galactomannan/dextran 2,000,000 systems were used for the purification, and the partition coefficients of alpha -galactosidase varied from 1.5 to 4.0. However, mass transport in these systems was poor due to the high viscosity of the employed polymers. Therefore, partitioning in polyethyleneglycol (PEG)/ sodium phosphate systems and the effect of sodium chloride upon the enzyme purification and the yield of a-galactosidase were also investigated. The purification achieved in a single-step was 5.7 with a recovery of 144% of a-galactosidase, possibly due to the removal of materials which inhibited a-galactosidase activity before the purification. The removal of the main protein contaminants and the highest yields were achieved in PEG 4,000/ sodium phosphate + 6% NaCl system at pH 5.0. Further purification by preparative on-exchange chromatography was also developed.
Subject: alpha-galactosidase
legume seeds
enzyme purification
aqueous two phase systems
Country: Brasil
Editor: Brazilian Soc Chemical Eng
Rights: aberto
Identifier DOI: 10.1590/S0104-66322000000400044
Date Issue: 2000
Appears in Collections:Unicamp - Artigos e Outros Documentos

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