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dc.contributor.CRUESPUniversidade Estadual de Campinaspt_BR
dc.typeArtigo de periódicopt_BR
dc.titlePurification and structural characterization of fengycin homologues produced by Bacillus subtilis LSFM-05 grown on raw glycerolpt_BR
dc.contributor.authorde Faria, AFpt_BR
dc.contributor.authorStefani, Dpt_BR
dc.contributor.authorVaz, BGpt_BR
dc.contributor.authorSilva, ISpt_BR
dc.contributor.authorGarcia, JSpt_BR
dc.contributor.authorEberlin, MNpt_BR
dc.contributor.authorGrossman, MJpt_BR
dc.contributor.authorAlves, OLpt_BR
dc.contributor.authorDurrant, LRpt_BR
unicamp.authorde Faria, Andreia Fonseca Silva, Isis Serrano Durrant, Lucia Regina Univ Estadual Campinas, Food Engn Fac, Dept Food Sci, BR-13083862 Campinas, SP, Brazilpt_BR
unicamp.authorStefani, Diego Vaz, Boniek Gontijo Garcia, Jerusa Simone Eberlin, Marcos N. Alves, Oswaldo Luiz Univ Estadual Campinas, Inst Chem, BR-13083970 Campinas, SP, Brazilpt_BR
unicamp.authorGrossman, Matthew James BioSage, Lawrenceville, NJ 08648 USApt_BR
dc.subjectBacillus subtilispt_BR
dc.subjectRaw glycerolpt_BR
dc.subjectESI FT-ICR mass spectrometrypt_BR
dc.subject.wosRhamnolipid Biosurfactant Productionpt_BR
dc.subject.wosPseudomonas-aeruginosa Lbipt_BR
dc.description.abstractRaw glycerol is a byproduct of biodiesel production that currently has low to negative value for biodiesel producers. One option for increasing the value of raw glycerol is to use it as a feedstock for microbial production. Bacillus subtilis LSFM 05 was used for the production of fengycin in a mineral medium containing raw glycerol as the sole carbon source. Fengycin was isolated by acid precipitation at pH 2 and purified by silica gel column chromatography and characterized using electrospray ionization (ESI) Fourier transform ion cyclotron resonance mass spectrometry (ESI FT-ICR MS) with collision-induced dissociation (CID). The mass spectrum revealed the presence of the ions of m/z 1,435.7, 1,449.9, 1,463.8, 1,477.8, 1,491.8 and 1,505.8, which were further fragmented by ESI-MS/MS. The CID profile showed the presence of a series of ions (m/z 1,080 and 966) and (m/z 1,108 and 994) that represented the different fengycin homologues A and B, respectively. Fengycin homologues A and B are variants that differ at position 6 of the peptide moiety, having either Ala or Val residues, respectively. Mass spectrometry analyses identified four fengycin A and three fengycin B variants with fatty acid components containing 14-17 carbons. These results demonstrate that raw glycerol can be used as feedstock to produce fengycin, and additional work should focus on the optimization of process conditions to increase
dc.relation.ispartofJournal Of Industrial Microbiology & Biotechnologypt_BR
dc.relation.ispartofabbreviationJ. Ind. Microbiol. Biotechnol.pt_BR
dc.publisherSpringer Heidelbergpt_BR
dc.identifier.citationJournal Of Industrial Microbiology & Biotechnology. Springer Heidelberg, v. 38, n. 7, n. 863, n. 871, 2011.pt_BR
dc.sourceWeb of Sciencept_BR
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
dc.description.sponsorship1Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt_BR
dc.description.sponsorship1Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt_BR
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