Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/58461
Type: Artigo de periódico
Title: Purification and inflammatory edema induced by two PLA(2) (Anch TX-I and Anch TX-II) from sea anemone Anthothoe chilensis (Actiniaria: Sagartiidae)
Author: Landucci, ECT
Dias, QC
Marangoni, FA
Vilca-Quispe, A
Valeriano-Zapana, JA
Torres-Huaco, FD
Martins-De-Souza, D
Marangoni, S
Ponce-Soto, LA
Abstract: The Anch TX-I and II PLA(2) were purified from Anthothoe chilensis (Lesson, 1830) from the extract of the anemone after only two chromatographic step using molecular exclusion chromatography (Sephadex G-75) and reverse phase HPLC on mu-Bondapak C18 column. Both PLA(2) showed a molecular mass of similar to 14 kDa determined by MALDI-TOF mass spectrometry and showed a high catalytic activity (data not showed). Although homologous with mammalian or snake venom group I PLA(2)s, Anch TX-I and II is sufficiently structurally different for the question of its placement into the existing PLA(2) classification scheme to arise. In addition, Anch TX-I and despite possessing many common structural features, also differ in some important structural properties. The amino acid sequence of both PLA(2) (Anch TX-I and III) showed high amino acid sequence identity with PLA(2) Rhopilema nomadica and Bunodosoma caissarum Cnidaria and PLA(2) of group III protein isolated from the Mexican lizard Heloderma horridum horridum and Heloderma suspectum. In addition, Anch TX-I and Anch TX-II showed high amino acid sequence identity with PLA(2) from group III also showed significant overall homology to bee Apis dorsata, Bombus terrestris and Bombus pennsylvanicus and PlA(2). We also investigated the in vivo edematogenic activity of Anch TX-I and Anch TX-II in a model of paw and skin edema in rats and observed that both are able to induce dose-dependent edema. (C) 2011 Elsevier Inc. All rights reserved.
Subject: Anthothoe chilensis
PLA(2)
Edematogenic
HPLC-RP
Rat paw edema
Country: EUA
Editor: Elsevier Science Inc
Rights: fechado
Identifier DOI: 10.1016/j.cbpb.2011.11.003
Date Issue: 2012
Appears in Collections:Unicamp - Artigos e Outros Documentos

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