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Type: Artigo de periódico
Title: Purification and characterization of an N-acetylglucosamine-binding lectin from Koelreuteria paniculata seeds and its effect on the larval development of Callosobruchus maculatus (Coleoptera : Bruchidae) and Anagasta kuehniella (Lepidoptera : Pyralidae)
Author: Macedo, MLR
Damico, DCS
Freire, MD
Toyama, MH
Marangoni, S
Novello, JC
Abstract: This study describes the purification of an N-acetylglucosamine-binding lectin from Koelreuteria paniculata seeds and its effects on the larval development of Callobruchus maculatus and Anagasta kuehniella. The lectin (KpLec) was characterized and isolated by gel filtration, affinity column, and reverse phase chromatography. SDS-PAGE indicated that this lectin is a dimer composed of subunits of 22 and 44 kDa. The N terminus exhibited 40% similarity with Urtiga dioica agglutinin. KpLec was tested for anti-insect activity against C. maculatus and A. kuehniella. With regard to C. maculatus, an artificial diet containing 0.7 and 1% KpLec produced LD50 and ED50 value, respectively. However, for A. kuenhiella, an artificial diet containing 0.65% KpLec produced an LD50, whereas 0.2% KpLec produced an ED50. The transformation of genes coding for this lectin could be useful in the development of insect resistance in important agricultural crops.
Subject: Koelreuteria panicuiata
N-acetylglucosamine binding
Callosobruchus maculatus
Anagasta kuehniella
Country: EUA
Editor: Amer Chemical Soc
Rights: fechado
Identifier DOI: 10.1021/jf034013i
Date Issue: 2003
Appears in Collections:Unicamp - Artigos e Outros Documentos

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