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Type: Artigo de periódico
Title: Proton dependence of tobacco mosaic virus dissociation by pressure
Author: Santos, JLR
Bispo, JAC
Landini, GF
Bonafe, CFS
Abstract: Tobacco mosaic virus (TMV) is an intensely studied model of viruses. This paper reports an investigation into the dissociation of TMV by pH and pressure up to 220 MPa. The viral solution (0.25 mg/ml) incubated at 277 K showed a significant decrease in light scattering with increasing pH, suggesting dissociation. This observation was confirmed by HPLC gel filtration and electron microscopy. The calculated volume change of dissociation (AV) decreased (absolute value) from - 49.7 ml/mol of subunit at pH 3.8 to - 21.7 ml/mol of subunit at pH 9.0. The decrease from pH 9.0 to 3.8 caused a stabilization of 14.1 kJ/mol of TMV subunit. The estimated proton release calculated from pressure-induced dissociation curves was 0.584 mol H+/mol of TMV subunit. These results suggest that the degree of virus inactivation by pressure and the immunogenicity of the inactivated structures can be optimized by modulating the surrounding pH, (C) 2004 Elsevier B.V. All rights reserved.
Subject: tobacco mosaic virus
pressure-induced dissociation
virus dissociation
Country: Holanda
Editor: Elsevier Science Bv
Rights: fechado
Identifier DOI: 10.1016/j.bpc.2004.04.003
Date Issue: 2004
Appears in Collections:Unicamp - Artigos e Outros Documentos

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